Western blot abreview for Anti-IRF5 antibody - ChIP Grade

Excellent
Abreviews
Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Mouse Cell lysate - whole cell (BMDM)
Specification
BMDM
Treatment
100 nM LPS, 24 h
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA with Tween-20- 0.01%
Dilution
1/2000

Secondary antibody

Name
Non-Abcam antibody was used: rabbit anti-goat secondory
Host species: Rabbit
Clonality: Polyclonal
Conjugation: IRDye­ 680
Dilution
1/10000

Detection

Detection method
licor odyssi
Bands
Specific: 58 kDa
Exposure
1 second(s)

Additional data

Additional Notes
Lane 1: marker Lane 2 : LPS induced BMDM

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Submitted Mar 20 2014

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