Western blot abreview for Anti-IRF5 antibody - ChIP Grade

Average
Abreviews
Application
Western blot
Loading amount
30 cells
Gel Running Conditions
Reduced Denaturing
Sample
Dog Cell lysate - whole cell (CPEK)
Specification
CPEK
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: southern biotech 6160-05
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL
Bands
Specific: ~70, ~74 kDa
Exposure
3 minute(s) and 0 second(s)
Positive control
AD293 human cells

Additional data

Additional Notes
Ladder: 170, 130, 95, 72, 56, 43, 34, 26, 17, 11, lane 1 is AD293, lane 2 is CPEK cells

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Submitted Sep 12 2014

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