Key features and details
- Rabbit polyclonal to IRF6
- Suitable for: ICC/IF, IHC-P
- Reacts with: Mouse, African green monkey
- Isotype: IgG
Product nameAnti-IRF6 antibody
See all IRF6 primary antibodies
DescriptionRabbit polyclonal to IRF6
Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, African green monkey
Synthetic peptide corresponding to Mouse IRF6 (C terminal).
Database link: P97431
- ICC: COS7 cells. IHC-P: Mouse small intestine.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: 100% PBS
0.2 µm filtered solution
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab275609 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/300 - 1/1000.|
|IHC-P||1/500 - 1/2000.|
FunctionProbable DNA-binding transcriptional activator. Key determinant of the keratinocyte proliferation-differentiation switch involved in appropriate epidermal development (By similarity). Plays a role in regulating mammary epithelial cell proliferation.
Tissue specificityExpressed in normal mammary epithelial cells. Expression is reduced or absent in breast carcinomas.
Involvement in diseaseDefects in IRF6 are a cause of van der Woude syndrome (VWS) [MIM:119300]; also known as lip-pit syndrome (LPS). It is an autosomal dominant developmental disorder characterized by lower lip pits, cleft lip and/or cleft palate. Penetrance is incomplete. Van der Woude and popliteal pterygium syndrome are allelic disorders.
Defects in IRF6 are the cause of popliteal pterygium syndrome (PPS) [MIM:119500]. PPS is an autosomal dominant developmental disorder characterized by cleft lip and/or cleft palate, and skin and genital anomalies. Penetrance is incomplete and expressivity is variable. It shows orofacial phenotypic similarities with van der Woude syndrome. Van der Woude and popliteal pterygium syndrome are allelic disorders.
Genetic variation in IRF6 is associated with non-syndromic orofacial cleft type 6 (OFC6) [MIM:608864]; also called non-syndromic cleft lip with or without cleft palate 6. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum.
Sequence similaritiesBelongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
modificationsPhosphorylated. Phosphorylation status depends on the cell cycle and is a signal for ubiquitination and proteasome-mediated degradation.
Cellular localizationNucleus. Cytoplasm. Translocates to nucleus in response to an activating signal.
- Information by UniProt
- Interferon regulatory factor 6 antibody
- IRF 6 antibody
- IRF-6 antibody
Immunocytochemical analysis of COS-7 (African green monkey kidney fibroblast-like cell line) cells labeling IRF6 with ab275609 at 1/1000 dilution at 4℃ overnight, followed by Goat Anti-rabbit IgG Alexa Fluor®488-conjugated secondary antibody (green). Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum prior incubation with ab275609. Positive staining was localized to cytoplasm and nucleus.
Immunohistochemical analysis of paraffin embedded mouse small intestine labeling IRF6 with ab275609 at 1/1000 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab275609 has not yet been referenced specifically in any publications.