Overview

  • Product name

    Anti-IRF6 antibody [OTI2A12]
    See all IRF6 primary antibodies
  • Description

    Mouse monoclonal [OTI2A12] to IRF6
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human IRF6. Produced in HEK293 cell (NP_006138)
    Database link: O14896

  • Positive control

    • Purchase matching WB positive control:Recombinant Human IRF6 protein
    • WB: HEK293T cells transfected with pCMV6-ENTRY IRF6 cDNA IHC-P: adenocarcinoma of Human colon tissue, Human kidney tissue, carcinoma of Human lung tissue, adenocarcinoma of Human endometrium tissue ICC/IF: COS7 cells transiently transfected by pCMV6-ENTRY IRF6 Flow Cyt: HEK293T cells transfected with pCMV6-ENTRY IRF6 overexpress plasmid

Properties

Applications

Our Abpromise guarantee covers the use of ab123880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 53 kDa.
IHC-P 1/150.
Flow Cyt 1/100.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100.

Target

  • Function

    Probable DNA-binding transcriptional activator. Key determinant of the keratinocyte proliferation-differentiation switch involved in appropriate epidermal development (By similarity). Plays a role in regulating mammary epithelial cell proliferation.
  • Tissue specificity

    Expressed in normal mammary epithelial cells. Expression is reduced or absent in breast carcinomas.
  • Involvement in disease

    Defects in IRF6 are a cause of van der Woude syndrome (VWS) [MIM:119300]; also known as lip-pit syndrome (LPS). It is an autosomal dominant developmental disorder characterized by lower lip pits, cleft lip and/or cleft palate. Penetrance is incomplete. Van der Woude and popliteal pterygium syndrome are allelic disorders.
    Defects in IRF6 are the cause of popliteal pterygium syndrome (PPS) [MIM:119500]. PPS is an autosomal dominant developmental disorder characterized by cleft lip and/or cleft palate, and skin and genital anomalies. Penetrance is incomplete and expressivity is variable. It shows orofacial phenotypic similarities with van der Woude syndrome. Van der Woude and popliteal pterygium syndrome are allelic disorders.
    Genetic variation in IRF6 is associated with non-syndromic orofacial cleft type 6 (OFC6) [MIM:608864]; also called non-syndromic cleft lip with or without cleft palate 6. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum.
  • Sequence similarities

    Belongs to the IRF family.
    Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
  • Post-translational
    modifications

    Phosphorylated. Phosphorylation status depends on the cell cycle and is a signal for ubiquitination and proteasome-mediated degradation.
  • Cellular localization

    Nucleus. Cytoplasm. Translocates to nucleus in response to an activating signal.
  • Information by UniProt
  • Database links

  • Alternative names

    • Interferon regulatory factor 6 antibody
    • IRF 6 antibody
    • IRF-6 antibody
    • IRF6 antibody
    • IRF6_HUMAN antibody
    • LPS antibody
    • OFC 6 antibody
    • OFC6 antibody
    • OTTHUMP00000034677 antibody
    • OTTHUMP00000034678 antibody
    • PIT antibody
    • PPS antibody
    • PPS1 antibody
    • VWS antibody
    • VWS1 antibody
    see all

Images

  • All lanes : Anti-IRF6 antibody [OTI2A12] (ab123880) at 1/2000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY IRF6 cDNA

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 53 kDa

  • Paraffin-embedded human colon adenocarcinoma tissue stained for IRF6 using ab123880 at 1/150 dilution in immunohistochemical analysis.

  • Paraffin-embedded human kidney tissue stained for IRF6 using ab123880 at 1/150 dilution in immunohistochemical analysis.

  • Paraffin-embedded human lung carcinoma tissue stained for IRF6 using ab123880 at 1/150 dilution in immunohistochemical analysis.

  • Paraffin-embedded human endometrium adenocarcinoma tissue stained for IRF6 using ab123880 at 1/150 dilution in immunohistochemical analysis.

  • COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected using pCMV6-ENTRY IRF6 stained for IRF6 (green) in ICC/IF.

  • Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either pCMV6-ENTRY IRF6 overexpress plasmid (Red), or empty vector control plasmid (Blue).

     

References

ab123880 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Granular Cell Layer)
Specification
Granular Cell Layer
Fixative
Formaldehyde
Permeabilization
Yes - 0.5% Triton X-100
Blocking step
Serum, plus monovalent Fab Fragment as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 18 2012

Question
Answer

Thank you for your phone call.

I have corrected the datasheet for ab10925. Thank you so much for pointing out the typos!

DISCOUNT CODE: xxx for ab115220
DISCOUNT CODE: xxx for ab123880
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab115220 and ab123880 in mouse. Eachcode will give you 1 free PRIMARY ANTIBODYbefore the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

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