Product nameAnti-IRF7 antibody
See all IRF7 primary antibodies
DescriptionRabbit polyclonal to IRF7
SpecificityThis antibody reacts with IRF7
Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
A synthetic peptide corresponding to 14 amino acids near the carboxy terminus of human IRF7
- 293 whole cell lysate and mouse spleen tissue HepG2 cells. Jurkat whole cells extract.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesIRF7 Antibody is affinity chromatography purified via peptide column.
- Epigenetics and Nuclear Signaling
- Domain Families
- HLH / Leucine Zipper
- Epigenetics and Nuclear Signaling
- Polymerase associated factors
- Pol II Transcription
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab62505 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 54 kDa).Can be blocked with Human IRF7 peptide (ab92594).|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 5 µg/ml.|
FunctionTranscriptional activator. Binds to the interferon-stimulated response element (ISRE) in IFN promoters and in the Q promoter (Qp) of EBV nuclear antigen 1 (EBNA1). Functions as a molecular switch for antiviral activity. Activated by phosphorylation in response to infection. Activation leads to nuclear retention, DNA binding, and derepression of transactivation ability.
Tissue specificityExpressed predominantly in spleen, thymus and peripheral blood leukocytes.
Sequence similaritiesBelongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
modificationsIn response to a viral infection, phosphorylated on the C-terminal serine cluster. Phosphorylation, and subsequent activation is inhibited by vaccinia virus protein E3.
TRAF6-mediated ubiquitination is required for IRF7 activation.
Cellular localizationNucleus. Cytoplasm. The phosphorylated and active form accumulates selectively in the nucleus.
- Information by UniProt
- IMD39 antibody
- Interferon regulatory factor 7 antibody
- Interferon regulatory factor 7H antibody
Lane 1 : Anti-IRF7 antibody (ab62505) at 0.5 µg/ml
Lane 2 : Anti-IRF7 antibody (ab62505) at 1 µg/ml
Lane 3 : Anti-IRF7 antibody (ab62505) at 2 µg/ml
All lanes : 293 whole cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 54 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of IRF7 expression in human spleen tissue with ab62505 at 5µg/ml.
ICC/IF image of ab62505 stained HepG2 cells (ab7900). The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum (ab7481) / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62505, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunofluorescence of IRF7 in Mouse Spleen cells using ab62505 at 20 ug/ml.
Immunohistochemistry of IRF7 in paraffin embedded mouse spleen tissue section with ab62505 antibody at 5 µg/ml.
IRF7 was immunoprecipitated using 0.5mg Jurkat whole cell extract (ab7899), 5µg of Rabbit polyclonal to IRF7 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab62505.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 49kDa: IRF7; non specific - 40kDa: We are unsure as to the identity of this extra band.
This product has been referenced in:
- Wang CH et al. Global Screening of Antiviral Genes that Suppress Baculovirus Transgene Expression in Mammalian Cells. Mol Ther Methods Clin Dev 6:194-206 (2017). Read more (PubMed: 28831401) »
- Katlinskaya YV et al. Type I Interferons Control Proliferation and Function of the Intestinal Epithelium. Mol Cell Biol 36:1124-35 (2016). Read more (PubMed: 26811327) »