Product nameAnti-IRF7 antibody [EPR23509-21] - BSA and Azide free
See all IRF7 primary antibodies
DescriptionRabbit monoclonal [EPR23509-21] to IRF7 - BSA and Azide free
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: ChIP,Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Human
Recombinant fragment within Human IRF7 aa 250 to the C-terminus. The exact sequence is proprietary.
Database link: Q92985
- WB: Jurkat treated with 100 nM IFN alpha for 18 hours, then added 10 µM MG-132 for another 6 hours, whole cell lysate. IP: Jurkat treated with 100 nM IFN alpha for 18 hours, then added 10 µM MG-132 for another 6 hours, whole cell lysate.
ab272225 is the carrier-free version of ab238137. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab272225 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.??
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
- Epigenetics and Nuclear Signaling
- Domain Families
- HLH / Leucine Zipper
- Epigenetics and Nuclear Signaling
- Polymerase associated factors
- Pol II Transcription
Our Abpromise guarantee covers the use of ab272225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 65, 45 kDa (predicted molecular weight: 54 kDa).|
|IP||Use at an assay dependent concentration.|
FunctionTranscriptional activator. Binds to the interferon-stimulated response element (ISRE) in IFN promoters and in the Q promoter (Qp) of EBV nuclear antigen 1 (EBNA1). Functions as a molecular switch for antiviral activity. Activated by phosphorylation in response to infection. Activation leads to nuclear retention, DNA binding, and derepression of transactivation ability.
Tissue specificityExpressed predominantly in spleen, thymus and peripheral blood leukocytes.
Sequence similaritiesBelongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
modificationsIn response to a viral infection, phosphorylated on the C-terminal serine cluster. Phosphorylation, and subsequent activation is inhibited by vaccinia virus protein E3.
TRAF6-mediated ubiquitination is required for IRF7 activation.
Cellular localizationNucleus. Cytoplasm. The phosphorylated and active form accumulates selectively in the nucleus.
- Information by UniProt
- IMD39 antibody
- Interferon regulatory factor 7 antibody
- Interferon regulatory factor 7H antibody
IRF7 was immunoprecipitated from 0.35 mg of Jurkat (treated with 100 nM IFN alpha for 18 hours, then added 10 μM MG-132 for another 6 hours) whole cell lysate with ab238137 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab238137 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366), was used as secondary antibody at 1/5000 dilution.
Lane 1: Jurkat (treated as above) whole cell lysate 10 μg (Input).
Lane 2: ab238137 IP in Jurkat (treated as above) whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab238137 in Jurkat (treated as above) whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 84 seconds.
65-kDa full length IRF7 and 45-kDa C-terminal cleaved IRF7 are observed (PMID: 23175366).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, glycerol, BSA and sodium azide (ab238137).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab272225 has not yet been referenced specifically in any publications.