Anti-IRF8 antibody (ab168808)
Key features and details
- Rabbit polyclonal to IRF8
- Suitable for: IP
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-IRF8 antibody
See all IRF8 primary antibodies -
Description
Rabbit polyclonal to IRF8 -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Rabbit, Chicken, Guinea pig, Cow, Dog, Turkey, Human, Pig, Chimpanzee, Duck, Lizard, Rhesus monkey, Gorilla, Chinese hamster, Orangutan -
Immunogen
Synthetic peptide corresponding to Mouse IRF8 aa 1-100.
Database link: P23611 -
Positive control
- RAW 264.7 whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab168808 was affinity purified using an epitope specific to IRF8 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168808 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Plays a negative regulatory role in cells of the immune system. -
Tissue specificity
Predominantly in lymphoid tissues. -
Sequence similarities
Belongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain. -
Post-translational
modificationsUbiquitinated. Ubiquitination by TRIM21 in macrophages, a process that is strongly increased upon interferon gamma stimulation, leds to the enhanced transcriptional activity of target cytokine genes. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 396385 Chicken
- Entrez Gene: 454294 Chimpanzee
- Entrez Gene: 100770390 Chinese hamster
- Entrez Gene: 614909 Cow
- Entrez Gene: 489673 Dog
- Entrez Gene: 101152979 Gorilla
- Entrez Gene: 3394 Human
- Entrez Gene: 15900 Mouse
see all -
Alternative names
- H ICSBP antibody
- H-ICSBP antibody
- HGNC:5358 antibody
see all
Images
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Detection of IRF8 in Immunoprecipitates of RAW 264.7 whole cell lysate (0.5 mg for IP, 20% of IP loaded) using ab168808 at 6 µg/mg lysate for IP. For WB detection an alternative anti-IRF8 antibody was used at 1 µg/ml.
Detection: Chemiluminescence with an exposure time of 30 seconds.Predicted MWt: 48 kDa.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab168808 has not yet been referenced specifically in any publications.