Key features and details
- Rabbit polyclonal to IRF8
- Suitable for: IP
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-IRF8 antibody
See all IRF8 primary antibodies
DescriptionRabbit polyclonal to IRF8
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Rabbit, Chicken, Guinea pig, Cow, Dog, Turkey, Human, Pig, Chimpanzee, Duck, Lizard, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
- RAW 264.7 whole cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab168808 was affinity purified using an epitope specific to IRF8 immobilized on solid support.
Our Abpromise guarantee covers the use of ab168808 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
FunctionSpecifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Plays a negative regulatory role in cells of the immune system.
Tissue specificityPredominantly in lymphoid tissues.
Sequence similaritiesBelongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
modificationsUbiquitinated. Ubiquitination by TRIM21 in macrophages, a process that is strongly increased upon interferon gamma stimulation, leds to the enhanced transcriptional activity of target cytokine genes.
- Information by UniProt
- H ICSBP antibody
- H-ICSBP antibody
- HGNC:5358 antibody
Detection of IRF8 in Immunoprecipitates of RAW 264.7 whole cell lysate (0.5 mg for IP, 20% of IP loaded) using ab168808 at 6 µg/mg lysate for IP. For WB detection an alternative anti-IRF8 antibody was used at 1 µg/ml.
Detection: Chemiluminescence with an exposure time of 30 seconds.
Predicted MWt: 48 kDa.
ab168808 has not yet been referenced specifically in any publications.