Anti-IRF8 antibody (ab245607)
Key features and details
- Rabbit polyclonal to IRF8
- Suitable for: IP, WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-IRF8 antibody
See all IRF8 primary antibodies -
Description
Rabbit polyclonal to IRF8 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide within Mouse IRF8 aa 374-424. The exact sequence is proprietary. NP_032346.1
Database link: P23611 -
Positive control
- WB: RAW 264.7 and L1210 whole cell lysate. IP: IRF8 IP in RAW 264.7 whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7-8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab245607 was affinity purified using an epitope specific to IRF8 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab245607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at 2-5 µg/mg of lysate.
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WB |
1/2000 - 1/10000.
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Notes |
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IP
Use at 2-5 µg/mg of lysate. |
WB
1/2000 - 1/10000. |
Target
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Function
Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Plays a negative regulatory role in cells of the immune system. -
Tissue specificity
Predominantly in lymphoid tissues. -
Sequence similarities
Belongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain. -
Post-translational
modificationsUbiquitinated. Ubiquitination by TRIM21 in macrophages, a process that is strongly increased upon interferon gamma stimulation, leds to the enhanced transcriptional activity of target cytokine genes. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 15900 Mouse
- SwissProt: P23611 Mouse
- Unigene: 334861 Mouse
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Alternative names
- H ICSBP antibody
- H-ICSBP antibody
- HGNC:5358 antibody
see all
Images
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Anti-IRF8 antibody (ab245607) at 0.1 µg/ml + L1210 whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 30 secondsPrepared using NETN lysis buffer.
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All lanes : Anti-IRF8 antibody (ab245607) at 0.1 µg/ml
Lane 1 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 50 µg
Lane 2 : RAW 264.7 whole cell lysate at 15 µg
Developed using the ECL technique.
Exposure time: 3 minutesPrepared using NETN lysis buffer.
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IRF8 was immunoprecipitated from RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab245607 used for IP at 3 µg per reaction. For WB 1 µg/ml.Lane 1: ab245607 IP in RAW 264.7 whole cell lysate.
Lane 2: Control IgG IP in RAW 264.7 whole cell lysate.
Chemiluminescence detection: 3 minutes.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab245607 has been referenced in 1 publication.
- Boto P et al. Zbtb46-dependent altered developmental program in embryonic stem cell-derived blood cell progenitors. Stem Cells N/A:N/A (2021). PubMed: 34058047