Overview

  • Product name

  • Description

    Rabbit polyclonal to IRF8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide within Mouse IRF8 aa 374-424. The exact sequence is proprietary. NP_032346.1
    Database link: P23611

  • Positive control

    • WB: RAW 264.7 and L1210 whole cell lysate. IP: IRF8 IP in RAW 264.7 whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7-8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab245607 was affinity purified using an epitope specific to IRF8 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab245607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
WB 1/2000 - 1/10000.

Target

  • Function

    Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Plays a negative regulatory role in cells of the immune system.
  • Tissue specificity

    Predominantly in lymphoid tissues.
  • Sequence similarities

    Belongs to the IRF family.
    Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
  • Post-translational
    modifications

    Ubiquitinated. Ubiquitination by TRIM21 in macrophages, a process that is strongly increased upon interferon gamma stimulation, leds to the enhanced transcriptional activity of target cytokine genes.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • H ICSBP antibody
    • H-ICSBP antibody
    • HGNC:5358 antibody
    • HICSBP antibody
    • ICSBP 1 antibody
    • ICSBP antibody
    • ICSBP1 antibody
    • Interferon consensus sequence binding protein 1 antibody
    • Interferon consensus sequence binding protein antibody
    • Interferon consensus sequence-binding protein antibody
    • Interferon regulatory factor 8 antibody
    • IRF 8 antibody
    • IRF-8 antibody
    • Irf8 antibody
    • IRF8_HUMAN antibody
    • MYLS antibody
    see all

Images

  • Anti-IRF8 antibody (ab245607) at 0.1 µg/ml + L1210 whole cell lysate at 50 µg

    Developed using the ECL technique.

    Exposure time: 30 seconds


    Prepared using NETN lysis buffer.

  • All lanes : Anti-IRF8 antibody (ab245607) at 0.1 µg/ml

    Lane 1 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 50 µg
    Lane 2 : RAW 264.7 whole cell lysate at 15 µg

    Developed using the ECL technique.

    Exposure time: 3 minutes


    Prepared using NETN lysis buffer.

  • IRF8 was immunoprecipitated from RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
    ab245607 used for IP at 3 µg per reaction. For WB 1 µg/ml.

    Lane 1: ab245607 IP in RAW 264.7 whole cell lysate.
    Lane 2: Control IgG IP in RAW 264.7 whole cell lysate.
    Chemiluminescence detection: 3 minutes.

References

ab245607 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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