Overview

  • Product name
  • Description
    Goat polyclonal to IRF8
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide: QRSFFRENQQITV, corresponding to C terminal amino acids 414-426 of Human ICSBP.

  • Positive control
    • K562 cell lysate
  • General notes
    Principal Names – ICSBP1; IRF8; ICSBP; IRF-8; interferon consensus sequence binding protein 1; H-ICSBP; interferon regulatory factor 8. Official Gene Symbol - ICSBP1. GenBank Accession Number – NP_002154. LocusLink ID - 3394 (human); 15900 (mouse). Gene Ontology terms - RNA polymerase II transcription factor activity; RNA polymerase II transcription factor activity, enhancer binding; immune response; negative regulation of transcription from Pol II promoter.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.30
    Constituents: 0.5% Tris, 0.5% BSA
  • Concentration information loading...
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).Can be blocked with Human IRF8 peptide (ab22947).

Detects a band of approximately 47 kDa in K562 lysate.

Target

  • Function
    Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Plays a negative regulatory role in cells of the immune system.
  • Tissue specificity
    Predominantly in lymphoid tissues.
  • Sequence similarities
    Belongs to the IRF family.
    Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
  • Post-translational
    modifications
    Ubiquitinated. Ubiquitination by TRIM21 in macrophages, a process that is strongly increased upon interferon gamma stimulation, leds to the enhanced transcriptional activity of target cytokine genes.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • H ICSBP antibody
    • H-ICSBP antibody
    • HGNC:5358 antibody
    • HICSBP antibody
    • ICSBP 1 antibody
    • ICSBP antibody
    • ICSBP1 antibody
    • Interferon consensus sequence binding protein 1 antibody
    • Interferon consensus sequence binding protein antibody
    • Interferon consensus sequence-binding protein antibody
    • Interferon regulatory factor 8 antibody
    • IRF 8 antibody
    • IRF-8 antibody
    • Irf8 antibody
    • IRF8_HUMAN antibody
    • MYLS antibody
    see all

Images

  • ab3428 at a 1µg/ml concentration staining ICSBP in K562 lysate (RIPA buffer, 30 µg total protein per lane, 1 hour incubation). Detected by Western blot (ECL).

    ab3428 at a 1µg/ml concentration staining ICSBP in K562 lysate (RIPA buffer, 30 µg total protein per lane, 1 hour incubation). Detected by Western blot (ECL).

References

ab3428 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your enquiry. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. If you have any more questions, please contact us again.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up