Overview

  • Product name

    Anti-IRF8 antibody [EPR20441]
    See all IRF8 primary antibodies
  • Description

    Rabbit monoclonal [EPR20441] to IRF8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human IRF8 aa 1-200. The exact sequence is proprietary.
    Database link: Q02556

  • Positive control

    • WB: THP-1 and Raji whole cell lysates; Human fetal spleen, tonsil and colon lysates. IHC-P: Human tonsil and non-hodgkin’s lymphoma tissues. IP: Raji whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20441
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab207418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IP 1/40.
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Plays a negative regulatory role in cells of the immune system.
  • Tissue specificity

    Predominantly in lymphoid tissues.
  • Sequence similarities

    Belongs to the IRF family.
    Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
  • Post-translational
    modifications

    Ubiquitinated. Ubiquitination by TRIM21 in macrophages, a process that is strongly increased upon interferon gamma stimulation, leds to the enhanced transcriptional activity of target cytokine genes.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • H ICSBP antibody
    • H-ICSBP antibody
    • HGNC:5358 antibody
    • HICSBP antibody
    • ICSBP 1 antibody
    • ICSBP antibody
    • ICSBP1 antibody
    • Interferon consensus sequence binding protein 1 antibody
    • Interferon consensus sequence binding protein antibody
    • Interferon consensus sequence-binding protein antibody
    • Interferon regulatory factor 8 antibody
    • IRF 8 antibody
    • IRF-8 antibody
    • Irf8 antibody
    • IRF8_HUMAN antibody
    • MYLS antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling IRF8 with ab207418 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on human tonsil (×200) [PMID: 18663414] [PMID: 16380510].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human non-hodgkin’s lymphoma tissue labeling IRF8 with ab207418 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on human non-hodgkin’s lymphoma.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • All lanes : Anti-IRF8 antibody [EPR20441] (ab207418) at 1/1000 dilution

    Lane 1 : THP-1 (Human monocytic leukemia cell line) whole cell lysate
    Lane 2 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 48 kDa
    Observed band size: 48 kDa


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-IRF8 antibody [EPR20441] (ab207418) at 1/1000 dilution

    Lane 1 : Human fetal spleen lysate
    Lane 2 : Human tonsil lysate
    Lane 3 : Human colon lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 48 kDa
    Observed band size: 48 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 30 seconds; Lane 2: 2 seconds; Lane 3: 15 seconds.

  • IRF8 was immunoprecipitated from 0.35 mg of Raji (Human Burkitt's lymphoma cell line) whole cell lysate with ab207418 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab207418 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Raji whole cell lysate 10 μg (Input).

    Lane 2: ab207418 IP in Raji whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207418 in Raji whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

References

This product has been referenced in:

  • Mohaghegh N  et al. NextPBM: a platform to study cell-specific transcription factor binding and cooperativity. Nucleic Acids Res N/A:N/A (2019). Read more (PubMed: 30657937) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human Tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 9 60' @ 95°C
Permeabilization
Yes - TX-100 20'
Specification
Human Tonsil
Blocking step
Goat IG Block as blocking agent for 8 minute(s) · Concentration: 100% · Temperature: 37°C
Fixative
10% NBF

Research Technician. Alex D

Verified customer

Submitted May 22 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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