RecombinantRabMAb

Recombinant Anti-IRF8 antibody [EPR20441] (ab207418)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF8 antibody [EPR20441] (ab207418)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF8 antibody [EPR20441] (ab207418)
  • Western blot - Anti-IRF8 antibody [EPR20441] (ab207418)
  • Western blot - Anti-IRF8 antibody [EPR20441] (ab207418)
  • Immunoprecipitation - Anti-IRF8 antibody [EPR20441] (ab207418)
  • Anti-IRF8 antibody [EPR20441] (ab207418)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20441] to IRF8
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-IRF8 antibody [EPR20441]
    See all IRF8 primary antibodies

  • Description

    Rabbit monoclonal [EPR20441] to IRF8

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: THP-1 and Raji whole cell lysates; Human fetal spleen, tonsil and colon lysates. IHC-P: Human tonsil and non-hodgkin’s lymphoma tissues. IP: Raji whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20441

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab207418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
IP
Human
WB
Human
Application Abreviews Notes
WB
1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IP
1/40.
IHC-P (1)
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IP
1/40.
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Plays a negative regulatory role in cells of the immune system.

  • Tissue specificity

    Predominantly in lymphoid tissues.

  • Sequence similarities

    Belongs to the IRF family.
    Contains 1 IRF tryptophan pentad repeat DNA-binding domain.

  • Post-translational
    modifications

    Ubiquitinated. Ubiquitination by TRIM21 in macrophages, a process that is strongly increased upon interferon gamma stimulation, leds to the enhanced transcriptional activity of target cytokine genes.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • H ICSBP antibody
      • H-ICSBP antibody
      • HGNC:5358 antibody
      • HICSBP antibody
      • ICSBP 1 antibody
      • ICSBP antibody
      • ICSBP1 antibody
      • Interferon consensus sequence binding protein 1 antibody
      • Interferon consensus sequence binding protein antibody
      • Interferon consensus sequence-binding protein antibody
      • Interferon regulatory factor 8 antibody
      • IRF 8 antibody
      • IRF-8 antibody
      • Irf8 antibody
      • IRF8_HUMAN antibody
      • MYLS antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF8 antibody [EPR20441] (ab207418)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF8 antibody [EPR20441] (ab207418)

      Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling IRF8 with ab207418 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on human tonsil (×200) [PMID: 18663414] [PMID: 16380510].

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF8 antibody [EPR20441] (ab207418)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF8 antibody [EPR20441] (ab207418)

      Immunohistochemical analysis of paraffin-embedded human non-hodgkin’s lymphoma tissue labeling IRF8 with ab207418 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on human non-hodgkin’s lymphoma.

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-IRF8 antibody [EPR20441] (ab207418)
      Western blot - Anti-IRF8 antibody [EPR20441] (ab207418)
      All lanes : Anti-IRF8 antibody [EPR20441] (ab207418) at 1/1000 dilution

      Lane 1 : THP-1 (Human monocytic leukemia cell line) whole cell lysate
      Lane 2 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 48 kDa
      Observed band size: 48 kDa


      Exposure time: 1 minute


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-IRF8 antibody [EPR20441] (ab207418)
      Western blot - Anti-IRF8 antibody [EPR20441] (ab207418)
      All lanes : Anti-IRF8 antibody [EPR20441] (ab207418) at 1/1000 dilution

      Lane 1 : Human fetal spleen lysate
      Lane 2 : Human tonsil lysate
      Lane 3 : Human colon lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

      Predicted band size: 48 kDa
      Observed band size: 48 kDa



      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: Lane 1: 30 seconds; Lane 2: 2 seconds; Lane 3: 15 seconds.

    • Immunoprecipitation - Anti-IRF8 antibody [EPR20441] (ab207418)
      Immunoprecipitation - Anti-IRF8 antibody [EPR20441] (ab207418)

      IRF8 was immunoprecipitated from 0.35 mg of Raji (Human Burkitt's lymphoma cell line) whole cell lysate with ab207418 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab207418 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Lane 1: Raji whole cell lysate 10 μg (Input).

      Lane 2: ab207418 IP in Raji whole cell lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207418 in Raji whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 1 second.

    • Anti-IRF8 antibody [EPR20441] (ab207418)
      Anti-IRF8 antibody [EPR20441] (ab207418)

    References (1)

    Publishing research using ab207418? Please let us know so that we can cite the reference in this datasheet.

    ab207418 has been referenced in 1 publication.

    • Mohaghegh N  et al. NextPBM: a platform to study cell-specific transcription factor binding and cooperativity. Nucleic Acids Res N/A:N/A (2019). PubMed: 30657937

    Customer reviews and Q&As

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IRF8 antibody [EPR20441]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Human Tonsil)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 9 60' @ 95°C
    Permeabilization
    Yes - TX-100 20'
    Specification
    Human Tonsil
    Blocking step
    Goat IG Block as blocking agent for 8 minute(s) · Concentration: 100% · Temperature: 37°C
    Fixative
    10% NBF
    Read More

    Research Technician. Alex D

    Verified customer

    Submitted May 22 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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