Key features and details
- Mouse monoclonal [mAbcam61750] to IRF8
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-IRF8 antibody [mAbcam61750]
See all IRF8 primary antibodies
DescriptionMouse monoclonal [mAbcam61750] to IRF8
Tested applicationsSuitable for: WB, Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
Recombinant fragment corresponding to Human IRF8 aa 100-250.
(Peptide available as
- This antibody gave a positive signal in Jurkat and K562 whole cell and nuclear lysates.
This antibody clone is manufactured by Abcam.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab61750 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 10 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa).|
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionSpecifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Plays a negative regulatory role in cells of the immune system.
Tissue specificityPredominantly in lymphoid tissues.
Sequence similaritiesBelongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
modificationsUbiquitinated. Ubiquitination by TRIM21 in macrophages, a process that is strongly increased upon interferon gamma stimulation, leds to the enhanced transcriptional activity of target cytokine genes.
- Information by UniProt
- H ICSBP antibody
- H-ICSBP antibody
- HGNC:5358 antibody
All lanes : Anti-IRF8 antibody [mAbcam61750] (ab61750) at 10 µg/ml
Lane 1 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lane 2 : K562 (Human erythromyeloblastoid leukemia cell line) Nuclear Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : Jurkat nuclear extract lysate (ab14844)
Lysates/proteins at 20 µg per lane.
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 13 kDa, 17 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
Overlay histogram showing K562 cells stained with ab61750 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab61750, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
ab61750 has been referenced in 1 publication.
- Suzuki M et al. Aberrant methylation and silencing of IRF8 expression in non-small cell lung cancer. Oncol Lett 8:1025-1030 (2014). IHC-P ; Human . PubMed: 25120651