Overview

  • Product name

  • Description

    Rabbit polyclonal to IRG1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • WB: LoVo whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab222417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/3000. Predicted molecular weight: 53 kDa.

Target

  • Sequence similarities

    Belongs to the prpD family.
  • Information by UniProt
  • Database links

  • Alternative names

    • ACOD1 antibody
    • Aconitate decarboxylase 1 antibody
    • Aconitate decarboxylase antibody
    • CAD antibody
    • Cis aconitate decarboxylase antibody
    • Immune responsive gene 1 protein homolog antibody
    • Immune-responsive gene 1 protein homolog antibody
    • Immunoresponsive 1 homolog (mouse) antibody
    • Irg1 antibody
    • IRG1_HUMAN antibody
    • OC116 antibody
    • OPTB1 antibody
    • TCTIRC7 antibody
    see all

Images

  • Anti-IRG1 antibody (ab222417) at 1/1000 dilution + LoVo (human colorectal adenocarcinoma cell line) whole cell lysate

    Predicted band size: 53 kDa

References

ab222417 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (Glioblastoma cells)
Gel Running Conditions
Reduced Denaturing (4 - 12%)
Loading amount
50000 cells
Specification
Glioblastoma cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C

Abcam user community

Verified customer

Submitted May 20 2019

Application
Western blot
Sample
Mouse Cell lysate - whole cell (BMDM lysate)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Treatment
10 ng/ml IFNy + 10ng/ml LPS or 20 ng/ml IL-4 or 0.4 nM palmitate for 24hrs
Specification
BMDM lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Sep 22 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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