RecombinantRabMAb

Recombinant Anti-IRG1 antibody [EPR22066] (ab222411)

Reviews (1) Submit a question
Immunoprecipitation - Anti-IRG1 antibody [EPR22066] (ab222411)
  • Western blot - Anti-IRG1 antibody [EPR22066] (ab222411)
  • Anti-IRG1 antibody [EPR22066] (ab222411)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22066] to IRG1
  • Suitable for: WB, IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-IRG1 antibody [EPR22066]
    See all IRG1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22066] to IRG1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment within Human IRG1 aa 250-450. The exact sequence is proprietary.
    Database link: A6NK06

  • Positive control

    • WB: RAW 264.7 (treated with 100 ng/ml lipopolysaccharide (LPS) for 4 hours) and THP-1 (treated with 100 ng/ml phorbol-12-myristate-13-acetate (PMA) for 72 hours, then with 1 µg/ml LPS after 52 hours) whole cell lysates. IP: THP-1 (treated with 100 ng/ml phorbol-12-myristate-13-acetate (PMA) for 72 hours, then with 1 µg/ml LPS added for 16 hours) whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab222411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 53 kDa).
IP 1/30.

Target

Images

  • Immunoprecipitation - Anti-IRG1 antibody [EPR22066] (ab222411)
    Immunoprecipitation - Anti-IRG1 antibody [EPR22066] (ab222411)

    IRG1 was immunoprecipitated from 0.35 mg of THP-1 (human monocytic leukemia cell line)(treated with 100 ng/ml phorbol-12-myristate-13-acetate (PMA) for 72 hours, then with 1 μg/ml LPS added for 16 hours) whole cell lysate with ab222411 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab222411 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

    Lane 1: THP-1 (treated with PMA/LPS) whole cell lysate, 10 μg (Input).
    Lane 2: ab222411 IP in THP-1 (treated with PMA/LPS) whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab222411 in THP-1 (treated with PMA/LPS) whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 8 seconds.

  • Western blot - Anti-IRG1 antibody [EPR22066] (ab222411)
    Western blot - Anti-IRG1 antibody [EPR22066] (ab222411)
    All lanes : Anti-IRG1 antibody [EPR22066] (ab222411) at 1/1000 dilution

    Lane 1 : Untreated RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 2 : RAW 264.7 (treated with 100 ng/ml lipopolysaccharide (LPS) for 4 hours) whole cell lysate
    Lane 3 : Untreated THP-1 (human monocytic leukemia cell line) whole cell lysate
    Lane 4 : THP-1 (treated with 100 ng/ml phorbol-12-myristate-13-acetate (PMA) for 72 hours, then with 1 µg/ml LPS after 52 hours) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 53 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lanes 1 & 2: 3 minutes.

    Lanes 3 & 4: 8 seconds.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 23610393 and PMID: 28699638).
    The protein is up-regulated after lipopolysaccharide (LPS) stimulation (PMID: 25640654 and PMID: 26679997).

  • Anti-IRG1 antibody [EPR22066] (ab222411)
    Anti-IRG1 antibody [EPR22066] (ab222411)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab222411? Please let us know so that we can cite the reference in this datasheet.

ab222411 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Western blot abreview for Anti-IRG1 antibody [EPR22066]

 Inconclusive
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (spinal cord)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
30 µg
Specification
spinal cord
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

qingyu

Verified customer

Submitted Sep 25 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com