Product nameAnti-IRG1 antibody [EPR22066]
See all IRG1 primary antibodies
DescriptionRabbit monoclonal [EPR22066] to IRG1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment within Human IRG1 aa 250-450. The exact sequence is proprietary.
Database link: A6NK06
- WB: RAW 264.7 (treated with 100 ng/ml lipopolysaccharide (LPS) for 4 hours) and THP-1 (treated with 100 ng/ml phorbol-12-myristate-13-acetate (PMA) for 72 hours, then with 1 µg/ml LPS after 52 hours) whole cell lysates. IP: THP-1 (treated with 100 ng/ml phorbol-12-myristate-13-acetate (PMA) for 72 hours, then with 1 µg/ml LPS added for 16 hours) whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab222411 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 53 kDa).|
Sequence similaritiesBelongs to the prpD family.
- Information by UniProt
- ACOD1 antibody
- Aconitate decarboxylase 1 antibody
- Aconitate decarboxylase antibody
IRG1 was immunoprecipitated from 0.35 mg of THP-1 (human monocytic leukemia cell line)(treated with 100 ng/ml phorbol-12-myristate-13-acetate (PMA) for 72 hours, then with 1 μg/ml LPS added for 16 hours) whole cell lysate with ab222411 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab222411 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.
Lane 1: THP-1 (treated with PMA/LPS) whole cell lysate, 10 μg (Input).
Lane 2: ab222411 IP in THP-1 (treated with PMA/LPS) whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab222411 in THP-1 (treated with PMA/LPS) whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 8 seconds.
All lanes : Anti-IRG1 antibody [EPR22066] (ab222411) at 1/1000 dilution
Lane 1 : Untreated RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2 : RAW 264.7 (treated with 100 ng/ml lipopolysaccharide (LPS) for 4 hours) whole cell lysate
Lane 3 : Untreated THP-1 (human monocytic leukemia cell line) whole cell lysate
Lane 4 : THP-1 (treated with 100 ng/ml phorbol-12-myristate-13-acetate (PMA) for 72 hours, then with 1 µg/ml LPS after 52 hours) whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 53 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Blocking and dilution buffer: 5% NFDM/TBST.
Lanes 1 & 2: 3 minutes.
Lanes 3 & 4: 8 seconds.
The molecular mass observed is consistent with what has been described in the literature (PMID: 23610393 and PMID: 28699638).
The protein is up-regulated after lipopolysaccharide (LPS) stimulation (PMID: 25640654 and PMID: 26679997).
ab222411 has not yet been referenced specifically in any publications.