Recombinant
RabMAb

Recombinant Anti-IRG1 antibody [EPR22066] (ab222411)

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Overview

  • Product name

    Anti-IRG1 antibody [EPR22066]
    See all IRG1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22066] to IRG1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment within Human IRG1 aa 250-450. The exact sequence is proprietary.
    Database link: A6NK06

  • Positive control

    • WB: RAW 264.7 (treated with 100 ng/ml lipopolysaccharide (LPS) for 4 hours) and THP-1 (treated with 100 ng/ml phorbol-12-myristate-13-acetate (PMA) for 72 hours, then with 1 µg/ml LPS after 52 hours) whole cell lysates. IP: THP-1 (treated with 100 ng/ml phorbol-12-myristate-13-acetate (PMA) for 72 hours, then with 1 µg/ml LPS added for 16 hours) whole cell lysate.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab222411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 53 kDa).
IP 1/30.

Target

Images

  • Immunoprecipitation - Anti-IRG1 antibody [EPR22066] (ab222411)
    Immunoprecipitation - Anti-IRG1 antibody [EPR22066] (ab222411)

    IRG1 was immunoprecipitated from 0.35 mg of THP-1 (human monocytic leukemia cell line)(treated with 100 ng/ml phorbol-12-myristate-13-acetate (PMA) for 72 hours, then with 1 μg/ml LPS added for 16 hours) whole cell lysate with ab222411 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab222411 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

    Lane 1: THP-1 (treated with PMA/LPS) whole cell lysate, 10 μg (Input).
    Lane 2: ab222411 IP in THP-1 (treated with PMA/LPS) whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab222411 in THP-1 (treated with PMA/LPS) whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 8 seconds.

  • Western blot - Anti-IRG1 antibody [EPR22066] (ab222411)
    Western blot - Anti-IRG1 antibody [EPR22066] (ab222411)
    All lanes : Anti-IRG1 antibody [EPR22066] (ab222411) at 1/1000 dilution

    Lane 1 : Untreated RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 2 : RAW 264.7 (treated with 100 ng/ml lipopolysaccharide (LPS) for 4 hours) whole cell lysate
    Lane 3 : Untreated THP-1 (human monocytic leukemia cell line) whole cell lysate
    Lane 4 : THP-1 (treated with 100 ng/ml phorbol-12-myristate-13-acetate (PMA) for 72 hours, then with 1 µg/ml LPS after 52 hours) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 53 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lanes 1 & 2: 3 minutes.

    Lanes 3 & 4: 8 seconds.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 23610393 and PMID: 28699638).
    The protein is up-regulated after lipopolysaccharide (LPS) stimulation (PMID: 25640654 and PMID: 26679997).

References

ab222411 has not yet been referenced specifically in any publications.

Publishing research using ab222411? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Western blot abreview for Anti-IRG1 antibody [EPR22066]

 Inconclusive
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (spinal cord)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
30 µg
Specification
spinal cord
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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qingyu

Verified customer

Submitted Sep 25 2019

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