Anti-IRGM antibody (ab118569)
Key features and details
- Rabbit polyclonal to IRGM
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IRGM antibody
See all IRGM primary antibodies -
Description
Rabbit polyclonal to IRGM -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to 14 amino acids from near the centre of Human IRGM.
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Positive control
- Human Colon and Brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab118569 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 20 kDa.
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 20 kDa. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Putative GTPase which is required for clearance of acute protozoan and bacterial infections. Functions in innate immune response probably through regulation of autophagy. May regulate proinflammatory cytokine production and prevent endotoxemia upon infection. May also play a role in macrophages adhesion and motility. -
Tissue specificity
Widely expressed (at protein level). Expressed in several tissues including colon, small bowel and peripheral blood leukocytes. -
Involvement in disease
Defects in IRGM are the cause of susceptibility to inflammatory bowel disease type 19 (IBD19) [MIM:612278]. A chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. -
Sequence similarities
Belongs to the interferon-inducible GTPase family. -
Cellular localization
Golgi apparatus membrane. Cell membrane. Cytoplasmic vesicle > phagosome membrane. Cytoplasmic vesicle > autophagosome membrane. Cell projection > phagocytic cup. Behaves like an integral membrane protein (By similarity). Recruited to the plasma membrane around forming phagocytic cups, it remains associated with maturing autophagosomes (By similarity). Preferentially associated with cis- and medial-Golgi. - Information by UniProt
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Database links
- Entrez Gene: 345611 Human
- Entrez Gene: 15944 Mouse
- Entrez Gene: 303090 Rat
- SwissProt: A1A4Y4 Human
- SwissProt: Q60766 Mouse
- SwissProt: Q6AYC2 Rat
- Unigene: 519680 Human
- Unigene: 652148 Human
see all -
Alternative names
- IFI1 antibody
- Iigp3 antibody
- Iipg3 antibody
see all
Images
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Human Colon (formalin-fixed, paraffin-embedded) stained with IRGM antibody ab118569 at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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Lane 1 : Anti-IRGM antibody (ab118569) at 1 µg/ml
Lane 2 : Anti-IRGM antibody (ab118569) at 2 µg/ml
All lanes : Human Brain lysate
Predicted band size: 20 kDa
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab118569 has been referenced in 2 publications.
- Cozzolino F et al. New label-free methods for protein relative quantification applied to the investigation of an animal model of Huntington Disease. PLoS One 15:e0238037 (2020). PubMed: 32886703
- Gobert AP et al. Hypusination Orchestrates the Antimicrobial Response of Macrophages. Cell Rep 33:108510 (2020). PubMed: 33326776