Key features and details
- Rabbit polyclonal to IRGM
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Do Not Freeze.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab69495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 21-46 kDa). please refer to databases and the literature for the expected molecular weights of species orthologues.|
FunctionPutative GTPase which is required for clearance of acute protozoan and bacterial infections. Functions in innate immune response probably through regulation of autophagy. May regulate proinflammatory cytokine production and prevent endotoxemia upon infection. May also play a role in macrophages adhesion and motility.
Tissue specificityWidely expressed (at protein level). Expressed in several tissues including colon, small bowel and peripheral blood leukocytes.
Involvement in diseaseDefects in IRGM are the cause of susceptibility to inflammatory bowel disease type 19 (IBD19) [MIM:612278]. A chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
Sequence similaritiesBelongs to the interferon-inducible GTPase family.
Cellular localizationGolgi apparatus membrane. Cell membrane. Cytoplasmic vesicle > phagosome membrane. Cytoplasmic vesicle > autophagosome membrane. Cell projection > phagocytic cup. Behaves like an integral membrane protein (By similarity). Recruited to the plasma membrane around forming phagocytic cups, it remains associated with maturing autophagosomes (By similarity). Preferentially associated with cis- and medial-Golgi.
- Information by UniProt
- IFI1 antibody
- Iigp3 antibody
- Iipg3 antibody
ab69495 has been referenced in 3 publications.
- Dabydeen SA et al. Comparison of tamoxifen and letrozole response in mammary preneoplasia of ER and aromatase overexpressing mice defines an immune-associated gene signature linked to tamoxifen resistance. Carcinogenesis 36:122-32 (2015). WB ; Mouse . PubMed: 25421723
- Springer HM et al. Irgm1 (LRG-47), a regulator of cell-autonomous immunity, does not localize to mycobacterial or listerial phagosomes in IFN-?-induced mouse cells. J Immunol 191:1765-74 (2013). WB . PubMed: 23842753
- Chang CP et al. Concanavalin A/IFN-Gamma Triggers Autophagy-Related Necrotic Hepatocyte Death through IRGM1-Mediated Lysosomal Membrane Disruption. PLoS One 6:e28323 (2011). WB, ICC/IF ; Mouse . PubMed: 22163006