Overview

  • Product name

  • Description

    Rabbit polyclonal to IRGM
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    A 14 amino acid peptide near the center of human IRGM

  • Positive control

    • Human brain lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab69495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 22163006
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 21-46 kDa). please refer to databases and the literature for the expected molecular weights of species orthologues.

Target

  • Function

    Putative GTPase which is required for clearance of acute protozoan and bacterial infections. Functions in innate immune response probably through regulation of autophagy. May regulate proinflammatory cytokine production and prevent endotoxemia upon infection. May also play a role in macrophages adhesion and motility.
  • Tissue specificity

    Widely expressed (at protein level). Expressed in several tissues including colon, small bowel and peripheral blood leukocytes.
  • Involvement in disease

    Defects in IRGM are the cause of susceptibility to inflammatory bowel disease type 19 (IBD19) [MIM:612278]. A chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
  • Sequence similarities

    Belongs to the interferon-inducible GTPase family.
  • Cellular localization

    Golgi apparatus membrane. Cell membrane. Cytoplasmic vesicle > phagosome membrane. Cytoplasmic vesicle > autophagosome membrane. Cell projection > phagocytic cup. Behaves like an integral membrane protein (By similarity). Recruited to the plasma membrane around forming phagocytic cups, it remains associated with maturing autophagosomes (By similarity). Preferentially associated with cis- and medial-Golgi.
  • Information by UniProt
  • Database links

  • Alternative names

    • IFI1 antibody
    • Iigp3 antibody
    • Iipg3 antibody
    • Immunity related GTPase family M protein 1 antibody
    • Immunity related GTPase family, M antibody
    • Immunity-related GTPase family M protein 1 antibody
    • Immunity-related GTPase family M protein antibody
    • Interferon inducible protein 1 antibody
    • Interferon-inducible protein 1 antibody
    • Irgm antibody
    • IRGM_HUMAN antibody
    • IRGM1 antibody
    • LPS-stimulated RAW 264.7 macrophage protein 47 homolog antibody
    • LRG 47 antibody
    • LRG-47 antibody
    • LRG-47-like protein antibody
    • LRG47 antibody
    • MGC149263 antibody
    • MGC149264 antibody
    see all

Images

  • Western blot analysis of IRGM in human brain lysate with ab69495 at (A) 1 and (B) 2 µg/mL.

References

This product has been referenced in:

  • Dabydeen SA  et al. Comparison of tamoxifen and letrozole response in mammary preneoplasia of ER and aromatase overexpressing mice defines an immune-associated gene signature linked to tamoxifen resistance. Carcinogenesis 36:122-32 (2015). WB ; Mouse . Read more (PubMed: 25421723) »
  • Springer HM  et al. Irgm1 (LRG-47), a regulator of cell-autonomous immunity, does not localize to mycobacterial or listerial phagosomes in IFN-?-induced mouse cells. J Immunol 191:1765-74 (2013). WB . Read more (PubMed: 23842753) »
See all 3 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Vielen Dank für Ihre Anfrage.

Der Austausch des ab69495 Anti-IRGM antibody gegen den ab65290 ist ueberhaupt kein Problem.

Ich eine kostenlose Ersatzlieferung fuer Sie in Auftrag gegeben. Sie hat die Referenznummer XXXXXX und sollte Montag bei Ihnen ankommen.

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Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse embryonic Fibroblasts)
Loading amount
150000 cells
Specification
Mouse embryonic Fibroblasts
Treatment
200U/ml interferon-gamma 24h
Gel Running Conditions
Reduced Denaturing (12% PA-SAS gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Apr 26 2012

Answer

Thank you for contacting Abcam.

We only know of one publication that has used ab69495, although there could be more that we are unaware of. The publication is below, also please note that we do place all of the information that we have about this antibody on the website, including any know publications:

Chang CP et al. Concanavalin A/IFN-Gamma Triggers Autophagy-Related Necrotic Hepatocyte Death through IRGM1-Mediated Lysosomal Membrane Disruption. PLoS One 6:e28323 (2011). WB, ICC/IF; Mouse. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=22163006&dopt=Abstract



We do not currently supply sample sizes of our antibodies but under our Abpromise we do guarantee them to work as stated on the datasheet. If you wish to try the antibody in an untested application or species then you could take advantage of our testing discount program. You can find more information about this from the link below:

https://www.abcam.com/index.htmlpageconfig=resource&rid=11998&viapagetrap=collaborationdiscount



If there is anything else I can help you with, please let me know.

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Answer

Thanks again for your question and for your patience. The peptide is now available as ab115829- https://www.abcam.com/IRGM-peptide-ab115829.html If you would like to forward your image and some protocol information, I would be happy to help identify the specific bands. Please let me know if you have any questions or if there is anything else you need.

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Answer

Thank you for contacting us with your question. I will be happy to check with the lab and see if we can add the blocking peptide for ab69495 to the catalog. If you would like, you can also send an image of your blot and some information about your protocol and I will help figure out which band may be IRGM. It may also be possible to reduce some of the non-specific bands by making a few changes to the protocol. I look forward to hearing from you, and I will let you know once I hear back from the lab about the blocking peptide.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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