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Would you be able to give us an estimate of normal Iron levels in serum and cultured cells?
Asked on Jan 25 2013
Normal serum Iron ~10-40 µM. Cells such as HeLa or cervical cells typically contain 1-0.9 pg iron/cell so 2 x 106 cells lysed in ~ 250 ul of assay buffer (Fe MW 55.85) should have about 4 – 8 nmol Fe per 50 ul test sample-possibly less if iron tightly bound and not released well be acid buffer (assay buffer)
Answered on Jan 25 2013