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During sample prep we are seeing cloudiness form but not blue color when using mouse liver samples. Samples are homogenized in Assay buffer with dounce homogenizer and spun at 16,000 x g as recommended. We have used straight attempted dilution of the samples 1/10 & 1/100. The kit is new and has been stored correctly.
Asked on May 22 2013
This is a common problem seen in liver and serum samples. Lipoproteins in the sample are the main culprits behind this turbidity.
For this purpose, we would recommend that to add 5 µl/well of 1 M SDS (28.8% or 288 mg/ml of SDS) to all their sample wells after step 4.2. Incubate for 30 min at 25 °C as mentioned in the datasheet and then follow the protocol. This SDS will clear up the turbidity (by dissolving any lipoproteins in the samples).
Answered on May 22 2013