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we would like to normalize the iron concentration with the protein content of our samples. Which methods (Lowry or Bradford) would you recommend?
Asked on Jul 09 2013
The buffer in this kit should be compatible with generic protein assays. We would prefer using the Bradford method. If the protein cannot be detected that way, we would recommend to have duplicates of the same number of cells. One of the samples can then be used for the protein assay and the second one can be lysed with any generic lysis buffer, so the protein quantity can be estimated.
Guillaume BoucherAbcam Scientific Support
Answered on Jul 09 2013