Overview

  • Product name
    Iron Stain Kit (Prussian Blue Stain)
    See all Iron kits
  • Product overview

    Iron Stain Kit (Prussian Blue stain) ab150674 is intended for use in the detection of ferric iron in tissues, blood smears, or bone marrow smears. Ferric iron is normally found in small amounts in bone marrow and the spleen. Abnormally large deposits may be seen in hemochromatosis and hemosiderosis.


    This iron stain kit is based on the Prussian Blue stain reaction in which ionic iron reacts with acid ferrocyanide producing a blue color.


    Iron stain / Prussian Blue stain protocol summary:
    - deparaffinize sections if necessary and rehydrate
    - incubate for 3 min in iron stain (mix of potassium ferrocyanide and hydrochloric acid)
    - rinse in water
    - counterstain in nuclear fast red
    - rinse in water
    - dehydrate in alcohol and mount  


    Other products for staining tissue sections


    Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

  • Notes

    Staining Interpretation

    Iron Bright Blue
    Nuclei Red
    Background   Pink

    Control Tissue: Spleen, Bone Marrow

Properties

Images

  • ab150674 Iron Stain Kit staining formalin-fixed-paraffin embedded human liver.

  • ab150674 (Iron stain) staining iron deposits (blue) in formalin fixed paraffin embedded normal human liver, counterstained with nuclear fast red (pink).

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • Staining of Human Kidney using ab150674 - Iron Stain Kit.

Protocols

References

This product has been referenced in:
  • Crippa S  et al. Bone marrow stromal cells from ß-thalassemia patients have impaired hematopoietic supportive capacity. J Clin Invest 129:1566-1580 (2019). Read more (PubMed: 30830876) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

iron staining of mouse liver

Good Good 4/5 (Ease of Use)
Abreviews
To demonstrate ferric iron in mouse liver tissue sections.

Abcam user community

Verified customer

Submitted May 04 2016

Improved staining time!

Excellent Excellent 5/5 (Ease of Use)
Abreviews
This kit has been a great time saver. With our home-made solutions, it took 30-60minutes for optimal staining of tissue samples. This kit cut staining time down to 3 minutes and the staining quality was equivalent!
It has been remarkably helpful in combining with long IHC assays, as it shortens the process. However, it also leeches out IHC chromogens when used subsequent to IHC, especially with Alk Phos reagents.

Ms. Shenna Washington

Verified customer

Submitted Aug 27 2014

Answer

Thank you for your inquiry.

I am happy to confirm that your protocol is suitable for this stain and that no changes are required.

I hope this information is reassuring and wish you good luck with your research.

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