Overview

  • Product name
    Anti-Iroquois homeobox protein 3 antibody
    See all Iroquois homeobox protein 3 primary antibodies
  • Description
    Rabbit polyclonal to Iroquois homeobox protein 3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Fr, ChIPmore details
  • Species reactivity
    Reacts with: Mouse, Chicken
    Predicted to work with: Human, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Mouse Iroquois homeobox protein 3.

    Read Abcam's proprietary immunogen policy (Peptide available as ab26906.)

  • Positive control
    • This antibody gave a positive signal in the following tissue lystaes: Mouse normal lung; Mouse normal brain; Mouse normal spinal cord and Mouse 14-day fetus.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 52, 55 kDa (predicted molecular weight: 52 kDa).
IHC-Fr 1/100.
ChIP Use at an assay dependent concentration. PubMed: 22992950

Target

  • Function
    Transcription factor. Involved in SHH-dependent neural patterning. Together with NKX2-2 and NKX6-1 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class I proteins of neuronal progenitor factors, which are repressed by SHH signals. Involved in the transcriptional repression of MNX1 in non-motor neuron cells.
  • Sequence similarities
    Belongs to the TALE/IRO homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI894186 antibody
    • Homeodomain protein IRXB1 antibody
    • Iroquois class homeodomain protein IRX 1 antibody
    • Iroquois homeobox 3 antibody
    • Iroquois homeobox protein 3 antibody
    • Iroquois-class homeodomain protein irx-3 antibody
    • IRX 1 antibody
    • irx3 antibody
    • IRX3_HUMAN antibody
    • IRXB1 antibody
    see all

Images

  • All lanes : Anti-Iroquois homeobox protein 3 antibody (ab25703) at 2 µg/ml

    Lane 1 : Mouse Fetus (14 Day Old) Tissue Lysate
    Lane 2 : Mouse lung normal tissue lysate - total protein (ab29297)
    Lane 3 : Brain (Mouse) Tissue Lysate
    Lane 4 : Spinal Cord (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 52 kDa
    Observed band size: 52,55 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:
  • Zou Y  et al. IRX3 Promotes the Browning of White Adipocytes and Its Rare Variants are Associated with Human Obesity Risk. EBioMedicine 24:64-75 (2017). Read more (PubMed: 28988979) »
  • Shekhar A  et al. Transcription factor ETV1 is essential for rapid conduction in the heart. J Clin Invest 126:4444-4459 (2016). IHC-Fr ; Mouse . Read more (PubMed: 27775552) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Embryonic ovary, lung, limb)
Specification
Embryonic ovary, lung, limb
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% Triton-X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Aug 02 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Chicken Tissue sections (Neural tube)
Specification
Neural tube
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 10%

Dr. Katrin Götz

Verified customer

Submitted Apr 23 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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