Overview

  • Product name

    Anti-Iroquois homeobox protein 3/IRX3 antibody
    See all Iroquois homeobox protein 3/IRX3 primary antibodies
  • Description

    Rabbit polyclonal to Iroquois homeobox protein 3/IRX3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Fr, ChIPmore details
  • Species reactivity

    Reacts with: Mouse, Chicken
    Predicted to work with: Human, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Mouse Iroquois homeobox protein 3/IRX3 aa 150-250 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab26905, ab26906)

  • Positive control

    • This antibody gave a positive signal in the following tissue lystaes: Mouse normal lung; Mouse normal brain; Mouse normal spinal cord and Mouse 14-day fetus.
  • General notes

     This product was previously labelled as Iroquois homeobox protein 3

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 52, 55 kDa (predicted molecular weight: 52 kDa).
IHC-Fr 1/100.
ChIP Use at an assay dependent concentration. PubMed: 22992950

Target

  • Function

    Transcription factor. Involved in SHH-dependent neural patterning. Together with NKX2-2 and NKX6-1 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class I proteins of neuronal progenitor factors, which are repressed by SHH signals. Involved in the transcriptional repression of MNX1 in non-motor neuron cells.
  • Sequence similarities

    Belongs to the TALE/IRO homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • AI894186 antibody
    • Homeodomain protein IRXB1 antibody
    • Iroquois class homeodomain protein IRX 1 antibody
    • Iroquois homeobox 3 antibody
    • Iroquois homeobox protein 3 antibody
    • Iroquois-class homeodomain protein irx-3 antibody
    • IRX 1 antibody
    • irx3 antibody
    • IRX3_HUMAN antibody
    • IRXB1 antibody
    see all

Images

  • All lanes : Anti-Iroquois homeobox protein 3/IRX3 antibody (ab25703) at 2 µg/ml

    Lane 1 : Mouse Fetus (14 Day Old) Tissue Lysate
    Lane 2 : Mouse lung normal tissue lysate - total protein (ab29297)
    Lane 3 : Brain (Mouse) Tissue Lysate
    Lane 4 : Spinal Cord (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 52 kDa
    Observed band size: 52,55 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Zou Y  et al. IRX3 Promotes the Browning of White Adipocytes and Its Rare Variants are Associated with Human Obesity Risk. EBioMedicine 24:64-75 (2017). Read more (PubMed: 28988979) »
  • Shekhar A  et al. Transcription factor ETV1 is essential for rapid conduction in the heart. J Clin Invest 126:4444-4459 (2016). IHC-Fr ; Mouse . Read more (PubMed: 27775552) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Embryonic ovary, lung, limb)
Specification
Embryonic ovary, lung, limb
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% Triton-X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Aug 02 2010

Application
Immunohistochemistry (Frozen sections)
Sample
Chicken Tissue sections (Neural tube)
Specification
Neural tube
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 10%
Fixative
Paraformaldehyde

Dr. Katrin Götz

Verified customer

Submitted Apr 23 2007

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