Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP327Y] to IRR
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Product nameAnti-IRR antibody [EP327Y]
See all IRR primary antibodies
DescriptionRabbit monoclonal [EP327Y] to IRR
Specificityab40782 recognises insulin receptor related receptor (IRR).
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human IRR aa 1-100 (N terminal). The exact sequence is proprietary.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab40782 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Predicted molecular weight: 80, 144 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionThis receptor probably binds an insulin related protein and has a tyrosine-protein kinase activity. It phosphorylates the insulin receptor substrates IRS-1 and IRS-2.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 3 fibronectin type-III domains.
Contains 1 protein kinase domain.
- Information by UniProt
- INSRR antibody
- INSRR_HUMAN antibody
- Insulin Receptor R antibody
All lanes : Anti-IRR antibody [EP327Y] (ab40782) at 1/2000 dilution
Lane 1 : PC12
Lane 2 : MCF7
Lane 3 : Hela
Lane 4 : SHSY5Y
Lysates/proteins at 10 µg per lane.
Predicted band size: 80, 144 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Additional bands at: 130 kDa, 37 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using ab40782.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab40782 has been referenced in 2 publications.
- Wang L et al. Exosomes derived from pancreatic cancer cells induce insulin resistance in C2C12 myotube cells through the PI3K/Akt/FoxO1 pathway. Sci Rep 7:5384 (2017). PubMed: 28710412
- Li Y et al. LRP4 mutations alter Wnt/beta-catenin signaling and cause limb and kidney malformations in Cenani-Lenz syndrome. Am J Hum Genet 86:696-706 (2010). WB ; Human . PubMed: 20381006