Overview

  • Product name

    Anti-IRR antibody [EP327Y]
    See all IRR primary antibodies
  • Description

    Rabbit monoclonal [EP327Y] to IRR
  • Host species

    Rabbit
  • Specificity

    ab40782 recognises insulin receptor related receptor (IRR).
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human IRR aa 1-100 (N terminal). The exact sequence is proprietary.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab40782 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 80, 144 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      This receptor probably binds an insulin related protein and has a tyrosine-protein kinase activity. It phosphorylates the insulin receptor substrates IRS-1 and IRS-2.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
      Contains 3 fibronectin type-III domains.
      Contains 1 protein kinase domain.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • INSRR antibody
      • INSRR_HUMAN antibody
      • Insulin Receptor R antibody
      • Insulin receptor related receptor precursor antibody
      • Insulin receptor-related protein alpha chain antibody
      • Insulin receptor-related protein beta chain antibody
      • insulin receptor-related receptor antibody
      • IR R antibody
      • IR related receptor antibody
      • IR-related receptor antibody
      • IRR antibody
      • Sir r antibody
      • Sirr antibody
      see all

    Images

    • All lanes : Anti-IRR antibody [EP327Y] (ab40782) at 1/2000 dilution

      Lane 1 : PC12
      Lane 2 : MCF7
      Lane 3 : Hela
      Lane 4 : SHSY5Y

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 80, 144 kDa
      Observed band size: 80 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 130 kDa, 37 kDa. We are unsure as to the identity of these extra bands.

    • Immunohistochemical analysis of paraffin-embedded human kidney tissue using ab40782.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Wang L  et al. Exosomes derived from pancreatic cancer cells induce insulin resistance in C2C12 myotube cells through the PI3K/Akt/FoxO1 pathway. Sci Rep 7:5384 (2017). Read more (PubMed: 28710412) »
    • Li Y  et al. LRP4 mutations alter Wnt/beta-catenin signaling and cause limb and kidney malformations in Cenani-Lenz syndrome. Am J Hum Genet 86:696-706 (2010). WB ; Human . Read more (PubMed: 20381006) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab40782.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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