Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2350(2)] to IRS1 + IRS2 (phospho S1101) - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Product nameAnti-IRS1 + IRS2 (phospho S1101) antibody [EPR2350(2)] - BSA and Azide free
See all IRS1 + IRS2 primary antibodies
DescriptionRabbit monoclonal [EPR2350(2)] to IRS1 + IRS2 (phospho S1101) - BSA and Azide free
This antibody only detects IRS1 phosphorylated at Serine 1101 and IRS2 phosphorylated at Serine 1149.
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human IRS1 + IRS2 aa 1050-1150 (phospho S1101). The exact sequence is proprietary. Immunogen is also 100% identical to Q9Y4H2 (IRS2) from aa 1144 to 1155 with phosphorylation at Serine 1149.
Database link: P35568
Ab250075 is the carrier-free version of ab178703. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab250075 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab250075 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 131 kDa.|
All lanes : Anti-IRS1 + IRS2 (phospho S1101 + S1149) antibody [EPR2350(2)] (ab178703) at 1/1000 dilution
Lane 1 : 293T cell lysate untreated
Lane 2 : 293T cell lysate treated with insulin
Lysates/proteins at 10 µg per lane.
Predicted band size: 131 kDa
This data was developed using ab178703, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab250075 has not yet been referenced specifically in any publications.