Recombinant Anti-IRS1 (phospho S1101) + IRS2 (phospho S1149) antibody [EPR2350(2)] - BSA and Azide free (ab250075)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2350(2)] to IRS1 (phospho S1101) + IRS2 (phospho S1149) - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-IRS1 (phospho S1101) + IRS2 (phospho S1149) antibody [EPR2350(2)] - BSA and Azide free -
Description
Rabbit monoclonal [EPR2350(2)] to IRS1 (phospho S1101) + IRS2 (phospho S1149) - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody only detects IRS1 phosphorylated at Serine 1101 and IRS2 phosphorylated at Serine 1149.
-
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
ab250075 is the carrier-free version of ab178703.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR2350(2) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Conjugation kits
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250075 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 131 kDa.
|
Notes |
---|
WB
Use at an assay dependent concentration. Predicted molecular weight: 131 kDa. |
Target
-
Cellular localization
IRS2: Cytoplasm > cytosol. -
Database links
- Entrez Gene: 3667 Human
- Entrez Gene: 8660 Human
- Entrez Gene: 16367 Mouse
- Entrez Gene: 384783 Mouse
- Entrez Gene: 25467 Rat
- Entrez Gene: 29376 Rat
- Omim: 147545 Human
- Omim: 600797 Human
see all
Images
-
All lanes : Anti-IRS1 (phospho S1101) + IRS2 (phospho S1149) antibody [EPR2350(2)] (ab178703) at 1/1000 dilution
Lane 1 : 293T cell lysate untreated
Lane 2 : 293T cell lysate treated with insulin
Lysates/proteins at 10 µg per lane.
Predicted band size: 131 kDaThis data was developed using ab178703, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab250075 has not yet been referenced specifically in any publications.