Anti-IRS1 antibody (ab131487)
Key features and details
- Rabbit polyclonal to IRS1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-IRS1 antibody
See all IRS1 primary antibodies -
Description
Rabbit polyclonal to IRS1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide conjugated to KLH, surrounding internal sequence amino acids 634-638 (P-M-S-P-K) of Human IRS1 (NP_005535.1).
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Positive control
- 293 cell extract; Human breast carcinoma tissue; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab131487 was purified by affinity chromatography using epitope specific peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131487 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/500 - 1/1000. Detects a band of approximately 180 kDa (predicted molecular weight: 131 kDa).
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IHC-P |
1/50 - 1/100.
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ICC/IF |
1/100 - 1/200.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 180 kDa (predicted molecular weight: 131 kDa). |
IHC-P
1/50 - 1/100. |
ICC/IF
1/100 - 1/200. |
Target
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Function
May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit. -
Involvement in disease
Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]. -
Sequence similarities
Contains 1 IRS-type PTB domain.
Contains 1 PH domain. -
Post-translational
modificationsSerine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
Phosphorylation of Tyr-896 is required for GRB2-binding. - Information by UniProt
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Database links
- Entrez Gene: 3667 Human
- Entrez Gene: 16367 Mouse
- Entrez Gene: 25467 Rat
- Omim: 147545 Human
- SwissProt: P35568 Human
- SwissProt: P35569 Mouse
- SwissProt: P35570 Rat
- Unigene: 471508 Human
see all -
Alternative names
- HIRS 1 antibody
- HIRS1 antibody
- Insulin receptor substrate 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of formaldehyde-fixed DB mice pancreas tissue staining with ab131487 at 1/50 dilution. Secondary antibody was ready to use HRP Immpress Goat anti-rabbit IGG. Samples were incubated with the primary antibody with SignalStain antibody diluent for 18 hours at 4°C. Blocking was done using 100% Bloxall for 20 minutes at 20°C. Heat mediated antigen retrieval with Tris-EDTA ph9
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Anti-IRS1 antibody (ab131487) at 1/500 dilution + 293 cell extract
Predicted band size: 131 kDa -
Immunofluorescent analysis of methanol-fixed HeLa cells labelling IRS1 with ab131487 at 1/100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human Breast carcinoma tissue labelling IRS1 with ab131487 at 1/50 dilution. The image on the right is treated with the synthesized peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab131487 has been referenced in 14 publications.
- Farini A et al. Microbiota dysbiosis influences immune system and muscle pathophysiology of dystrophin-deficient mice. EMBO Mol Med 15:e16244 (2023). PubMed: 36533294
- Marfella R et al. Sodium/glucose cotransporter 2 (SGLT2) inhibitors improve cardiac function by reducing JunD expression in human diabetic hearts. Metabolism 127:154936 (2022). PubMed: 34801581
- Webert L et al. Analysis of the Glucose-Dependent Transcriptome in Murine Hypothalamic Cells. Cells 11:N/A (2022). PubMed: 35203289
- Xu W et al. Anti-inflammatory activities of puerarin in high-fat diet-fed rats with streptozotocin-induced gestational diabetes mellitus. Mol Biol Rep 47:7537-7546 (2020). PubMed: 32946041
- Li L et al. Salvianolic acid B prevents body weight gain and regulates gut microbiota and LPS/TLR4 signaling pathway in high-fat diet-induced obese mice. Food Funct 11:8743-8756 (2020). PubMed: 32955050