Anti-IRS1 antibody (ab131487)

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Overview

  • Product name

    Anti-IRS1 antibody
    See all IRS1 primary antibodies

  • Description

    Rabbit polyclonal to IRS1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide conjugated to KLH, surrounding internal sequence amino acids 634-638 (P-M-S-P-K) of Human IRS1 (NP_005535.1).

  • Positive control

    • 293 cell extract; Human breast carcinoma tissue; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab131487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 180 kDa (predicted molecular weight: 131 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/200.

Target

  • Function

    May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.

  • Involvement in disease

    Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].

  • Sequence similarities

    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.

  • Post-translational
    modifications

    Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
    Phosphorylation of Tyr-896 is required for GRB2-binding.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • HIRS 1 antibody
    • HIRS1 antibody
    • Insulin receptor substrate 1 antibody
    • IRS 1 antibody
    • IRS-1 antibody
    • IRS1 antibody
    • IRS1_HUMAN antibody
    • OTTHUMP00000164234 antibody
    see all

Images

  • Western blot - Anti-IRS1 antibody (ab131487)
    Western blot - Anti-IRS1 antibody (ab131487)
    Anti-IRS1 antibody (ab131487) at 1/500 dilution + 293 cell extract

    Predicted band size: 131 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-IRS1 antibody (ab131487)
    Immunocytochemistry/ Immunofluorescence - Anti-IRS1 antibody (ab131487)
    Immunofluorescent analysis of methanol-fixed HeLa cells labelling IRS1 with ab131487 at 1/100 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1 antibody (ab131487)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1 antibody (ab131487)
    Immunohistochemical analysis of paraffin-embedded Human Breast carcinoma tissue labelling IRS1 with ab131487 at 1/50 dilution. The image on the right is treated with the synthesized peptide.

References

This product has been referenced in:

  • Song C  et al. Sericin enhances the insulin-PI3K/AKT signaling pathway in the liver of a type 2 diabetes rat model. Exp Ther Med 16:3345-3352 (2018). Read more (PubMed: 30250521) »
  • Deng Z  et al. Infusion of adipose-derived mesenchymal stem cells inhibits skeletal muscle mitsugumin 53 elevation and thereby alleviates insulin resistance in type 2 diabetic rats. Mol Med Rep 17:8466-8474 (2018). Read more (PubMed: 29693163) »
See all 4 Publications for this product

Publishing research using ab131487? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Western blot abreview for Anti-IRS1 antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (EMT6 cells)
Gel Running Conditions
Reduced Denaturing (4-20% Tris Glycin gel, semi dry transfer)
Loading amount
50 µg
Specification
EMT6 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

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Submitted Jul 06 2018

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