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    irs1-antibody-ab131487.pdf

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Anti-IRS1 antibody (ab131487)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (7)

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Western blot - Anti-IRS1 antibody (ab131487)
  • Immunocytochemistry/ Immunofluorescence - Anti-IRS1 antibody (ab131487)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1 antibody (ab131487)

Key features and details

  • Rabbit polyclonal to IRS1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Knockout
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Human IRS1 knockout HEK-293T cell line (ab267320)
Protein
Product image
Recombinant Human IRS1 protein (ab70538)

View more associated products

Overview

  • Product name

    Anti-IRS1 antibody
    See all IRS1 primary antibodies
  • Description

    Rabbit polyclonal to IRS1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide conjugated to KLH, surrounding internal sequence amino acids 634-638 (P-M-S-P-K) of Human IRS1 (NP_005535.1).

  • Positive control

    • 293 cell extract; Human breast carcinoma tissue; HeLa cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride

    PBS without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab131487 was purified by affinity chromatography using epitope specific peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Signal Transduction
    • Adapters
    • Cytoplasmic
    • Signal Transduction
    • Growth Factors/Hormones
    • Insulin / Insulin-like
    • Neuroscience
    • Neurology process
    • Metabolism
    • Cancer
    • Signal transduction
    • Other
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Heart disease

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human IRS1 protein (ab70538)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab131487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Human
Application Abreviews Notes
WB (1)
1/500 - 1/1000. Detects a band of approximately 180 kDa (predicted molecular weight: 131 kDa).
IHC-P
1/50 - 1/100.
ICC/IF
1/100 - 1/200.
Notes
WB
1/500 - 1/1000. Detects a band of approximately 180 kDa (predicted molecular weight: 131 kDa).
IHC-P
1/50 - 1/100.
ICC/IF
1/100 - 1/200.

Target

  • Function

    May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
  • Involvement in disease

    Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
  • Sequence similarities

    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • Post-translational
    modifications

    Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
    Phosphorylation of Tyr-896 is required for GRB2-binding.
  • Target information above from: UniProt accession P35568 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3667 Human
    • Omim: 147545 Human
    • SwissProt: P35568 Human
    • Unigene: 471508 Human
    • Alternative names

      • HIRS 1 antibody
      • HIRS1 antibody
      • Insulin receptor substrate 1 antibody
      • IRS 1 antibody
      • IRS-1 antibody
      • IRS1 antibody
      • IRS1_HUMAN antibody
      • OTTHUMP00000164234 antibody
      see all

    Images

    • Western blot - Anti-IRS1 antibody (ab131487)
      Western blot - Anti-IRS1 antibody (ab131487)
      Anti-IRS1 antibody (ab131487) at 1/500 dilution + 293 cell extract

      Predicted band size: 131 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-IRS1 antibody (ab131487)
      Immunocytochemistry/ Immunofluorescence - Anti-IRS1 antibody (ab131487)
      Immunofluorescent analysis of methanol-fixed HeLa cells labelling IRS1 with ab131487 at 1/100 dilution.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1 antibody (ab131487)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1 antibody (ab131487)
      Immunohistochemical analysis of paraffin-embedded Human Breast carcinoma tissue labelling IRS1 with ab131487 at 1/50 dilution. The image on the right is treated with the synthesized peptide.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (7)

    Publishing research using ab131487? Please let us know so that we can cite the reference in this datasheet.

    ab131487 has been referenced in 7 publications.

    • Zhang Y  et al. Hyperinsulinemia Can Cause Kidney Disease in the IGT Stage of OLETF Rats via the INS/IRS-1/PI3-K/Akt Signaling Pathway. J Diabetes Res 2019:4709715 (2019). PubMed: 31737684
    • Wang Y  et al. Silencing of SAA1 inhibits palmitate- or high-fat diet induced insulin resistance through suppression of the NF-?B pathway. Mol Med 25:17 (2019). PubMed: 31060494
    • Xuguang H  et al. Hesperidin ameliorates insulin resistance by regulating the IRS1-GLUT2 pathway via TLR4 in HepG2 cells. Phytother Res 33:1697-1705 (2019). PubMed: 31074547
    • Song C  et al. Sericin enhances the insulin-PI3K/AKT signaling pathway in the liver of a type 2 diabetes rat model. Exp Ther Med 16:3345-3352 (2018). PubMed: 30250521
    • Deng Z  et al. Infusion of adipose-derived mesenchymal stem cells inhibits skeletal muscle mitsugumin 53 elevation and thereby alleviates insulin resistance in type 2 diabetic rats. Mol Med Rep 17:8466-8474 (2018). PubMed: 29693163
    • Wang T  et al. Involvement of Insulin Signaling Disturbances in Bisphenol A-Induced Alzheimer's Disease-like Neurotoxicity. Sci Rep 7:7497 (2017). PubMed: 28790390
    • Zhao J  et al. Low-dose testosterone alleviates vascular damage caused by castration in male rats in puberty via modulation of the PI3K/AKT signaling pathway. Mol Med Rep 14:2518-26 (2016). WB, IHC-P ; Rat . PubMed: 27485938

    Customer reviews and Q&As

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    Western blot abreview for Anti-IRS1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (EMT6 cells)
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris Glycin gel, semi dry transfer)
    Loading amount
    50 µg
    Specification
    EMT6 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 06 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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