Key features and details
- Rabbit polyclonal to IRS1
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-IRS1 antibody
See all IRS1 primary antibodies
DescriptionRabbit polyclonal to IRS1
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human IRS1 aa 750-800. The exact sequence is proprietary. NP_005535.1
Database link: P35568
- IP: HeLa whole cell lysate.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245313 was affinity purified using an epitope specific to IRS1 immobilized on solid support.
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab245313 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
Use at 2-5 µg/mg of lysate.
FunctionMay mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
Involvement in diseasePolymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
Sequence similaritiesContains 1 IRS-type PTB domain.
Contains 1 PH domain.
modificationsSerine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
Phosphorylation of Tyr-896 is required for GRB2-binding.
- Information by UniProt
- HIRS 1 antibody
- HIRS1 antibody
- Insulin receptor substrate 1 antibody
IRS1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245313 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using a different Anti-IRS1 antibody at 1 µg/ml.
Lane 1: ab245313 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245313 has not yet been referenced specifically in any publications.