Anti-IRS1 antibody (ab52167)

Rabbit polyclonal IRS1 antibody. Validated in WB, ELISA, IHC, ICC/IF and tested in Mouse, Rat, Human. Cited in 21 publication(s). Immunogen corresponding to synthetic peptide.

Overview

  • Product name

  • Description

    Rabbit polyclonal to IRS1
  • Host species

    Rabbit
  • Specificity

    ab52167 detects endogenous levels of total IRS1 protein.
  • Tested applications

    Suitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic non-phosphopeptide derived from human IRS1 around the phosphorylation site of serine 307 (T-E-S-I-T).

  • Positive control

    • Human breast carcinoma tissue and A431 cell extarcts.

Properties

Applications

Our Abpromise guarantee covers the use of ab52167 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 132 kDa.
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 5 µg/ml.

Target

  • Function

    May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
  • Involvement in disease

    Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
  • Sequence similarities

    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • Post-translational
    modifications

    Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
    Phosphorylation of Tyr-896 is required for GRB2-binding.
  • Information by UniProt
  • Database links

  • Alternative names

    • HIRS 1 antibody
    • HIRS1 antibody
    • Insulin receptor substrate 1 antibody
    • IRS 1 antibody
    • IRS-1 antibody
    • IRS1 antibody
    • IRS1_HUMAN antibody
    • OTTHUMP00000164234 antibody
    see all

Images

  • All lanes : Anti-IRS1 antibody (ab52167) at 1/500 dilution

    Lane 1 : A431 cell extract
    Lane 2 : A431 cell extract with immunising peptide


    Lysates/proteins at 5 µg per lane.

    Predicted band size: 132 kDa
    Observed band size: >175 kDa
    why is the actual band size different from the predicted?

  • ab52167 at 1/50 dilution staining IRS1 in human breast carcinoma by Immunohistochemsitry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
  • ICC/IF image of ab52167 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52167, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Ren Y  et al. CRNDE promotes cell tongue squamous cell carcinoma cell growth and invasion through suppressing miR-384. J Cell Biochem 120:155-163 (2019). Read more (PubMed: 30242873) »
  • Hwang JH  et al. TAZ couples Hippo/Wnt signalling and insulin sensitivity through Irs1 expression. Nat Commun 10:421 (2019). Read more (PubMed: 30679431) »
See all 26 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for contacting us.

I have assignedyou to our Technical Support in Honkkong office (mailto:hk.technical@abcam.com)


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your enquiry.

For pricing and related information regarding the purchase of ab52167, please contact our distributor in India at:
https://www.abcam.com/index.html?pageconfig=contactus&howToOrderCountry=IN

I hope this is helpful. Please contact us again if you have any further questions.

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Answer

Thank you for your enquiry. I am sorry to hear this vial of ab52167 is giving you trouble. We don't have a specificIHC-P protocol available for this antibody. But If you could, please complete the formbelow so I can get an idea of your protocol and data. This will make it easier for me to offer troubleshooting tips. If the antibody continues toperform poorly after taking into account anytips, I would behappyto offer a replacement, credit or refund.

Thanks in advance for the additional information!

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1) Abcam product code ab



2) Abcam order reference number or product batch number



3) Description of the problem



4) Sample preparation:

Species

Type of sample: Fresh frozen sections, perfusion fixed frozen sections, PFA/formalin fixed paraffin embedded sections, cells in culture, other:

Sample preparation

Positive control

Negative control



5) Fixation step

Yes/No

If yes: Fixative agent and concentration

Fixation time

Fixation temperature



6) Antigen retrieval method



7) Permeabilization method:

Did you do a permeabilization step (details please) or add permeabilizing agent in any dilution buffers?

Permeabilizing agent and concentration:





8) Blocking agent (eg BSA, serum…):

Concentration

Blocking time

Blocking temperature



9) Endogenous peroxidases blocked?

Endogenous biotins blocked?



10) Primary antibody (If more than one was used, describe in “additional notes”) :

Concentration or dilution

Diluent buffer

Incubation time



11) Secondary antibody:

Species:

Reacts against:

Concentration or dilution

Diluent buffer

Incubation time

Fluorochrome or enzyme conjugate



12) Washing after primary and secondary antibodies:

Buffer

Number of washes



13) Detection method



14) How many times have you run this staining?

Do you obtain the same results every time?

What steps have you altered to try and optimize the use of this antibody?



Document attachment: Attaching images of your IHC is strongly recommended and can greatly speed up our investigation of your problem.





Please send this questionnaire by e-mail by cutting and pasting the text in your e-mail (no attachments other than images) to technical@abcam.com.

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Question
Answer

Thank you for your call today and for letting us know about the problem with ab52167. As we discussed, I am sending a vial of ab653 and I expect it to arrive next week. I found out that we don't ship to Canada on Saturdays, so the order will go out on Monday. Please keep me updated about the results using this replacement antibody, and let me know if you have any questions. Have a great weekend!

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