Anti-IRS1 antibody (ab65746)

Submit a review Submit a question
Western blot - Anti-IRS1 antibody (ab65746)

    Key features and details

    • Sheep polyclonal to IRS1
    • Suitable for: WB
    • Reacts with: Mouse, Rat, Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-IRS1 antibody
      See all IRS1 primary antibodies

    • Description

      Sheep polyclonal to IRS1

    • Host species

      Sheep

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Mouse, Rat, Human
      Predicted to work with: Chicken, Pig

    • Immunogen

      Synthetic peptide:

      CSISFQKQPEDRQ

      conjugated to KLH, corresponding to C terminal amino acids 1231-1242 of Human IRS1
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • Positive control

      • CHO-IR cell extracts.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Associated products

    Applications

    Our Abpromise guarantee covers the use of ab65746 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use a concentration of 2 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 132 kDa).

    Target

    • Function

      May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.

    • Involvement in disease

      Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].

    • Sequence similarities

      Contains 1 IRS-type PTB domain.
      Contains 1 PH domain.

    • Post-translational
      modifications

      Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
      Phosphorylation of Tyr-896 is required for GRB2-binding.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • HIRS 1 antibody
      • HIRS1 antibody
      • Insulin receptor substrate 1 antibody
      • IRS 1 antibody
      • IRS-1 antibody
      • IRS1 antibody
      • IRS1_HUMAN antibody
      • OTTHUMP00000164234 antibody
      see all

    Images

    References (0)

    Publishing research using ab65746? Please let us know so that we can cite the reference in this datasheet.

    ab65746 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab65746.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com