WBmore details Unsuitable for:
Flow Cyt,ICC/IF,IHC-P or IP
A synthetic peptide corresponding to residues near the C-terminus of human IRS1. Phosphorylated at tyrosine 896.
293 cell lysate.
This antibody was re-validated and was confirmed to recognize total IRS1. The name was changed from Anti-IRS1 (phospho Y896) antibody [EP260Y] to Anti-IRS1 antibody [EP260Y] on May 1st, 2018.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Detects a band of approximately 170 kDa (predicted molecular weight: 132 kDa).
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
Involvement in disease
Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor. Phosphorylation of Tyr-896 is required for GRB2-binding.
Wang T et al. Involvement of Insulin Signaling Disturbances in Bisphenol A-Induced Alzheimer's Disease-like Neurotoxicity. Sci Rep7:7497 (2017).
Read more (PubMed: 28790390) »
Liu Z et al. Adiponectin reduces ER stress-induced apoptosis through PPARa transcriptional regulation of ATF2 in mouse adipose. Cell Death Dis7:e2487 (2016).
Read more (PubMed: 27882945) »