Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
RecombinantRabMAb

Recombinant Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)

Submit a review Submit a question
Western blot - Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)
  • Immunocytochemistry - Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)
  • Western blot - Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)
  • Flow Cytometry (Intracellular) - Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)
  • Western blot - Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)
  • Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP263Y] to IRS1 - BSA and Azide free
  • Suitable for: ICC, IHC-P, WB, Flow Cyt (Intra)
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-IRS1 antibody [EP263Y] - BSA and Azide free
    See all IRS1 primary antibodies

  • Description

    Rabbit monoclonal [EP263Y] to IRS1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, WB, Flow Cyt (Intra)more details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247286 is the carrier-free version of ab40777.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab247286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
WB
Use at an assay dependent concentration. Detects a band of approximately 170 kDa (predicted molecular weight: 130 kDa).
Flow Cyt (Intra)
Use at an assay dependent concentration.
Notes
ICC
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
WB
Use at an assay dependent concentration. Detects a band of approximately 170 kDa (predicted molecular weight: 130 kDa).
Flow Cyt (Intra)
Use at an assay dependent concentration.
Application notes
Is unsuitable for IP.

Target

  • Function

    May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.

  • Involvement in disease

    Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].

  • Sequence similarities

    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.

  • Post-translational
    modifications

    Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
    Phosphorylation of Tyr-896 is required for GRB2-binding.
  • Information by UniProt

  • Database links

    • Alternative names

      • HIRS 1 antibody
      • HIRS1 antibody
      • Insulin receptor substrate 1 antibody
      • IRS 1 antibody
      • IRS-1 antibody
      • IRS1 antibody
      • IRS1_HUMAN antibody
      • OTTHUMP00000164234 antibody
      see all

    Images

    • Western blot - Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)
      Western blot - Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)
      Anti-IRS1 antibody [EP263Y] (ab40777) at 1/10000 dilution (purified) + HEK293 whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 130 kDa
      Observed band size: 170 kDa why is the actual band size different from the predicted?



      This data was developed using ab40777, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)

      This data was developed using ab40777, the same antibody clone in a different buffer formulation.

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue labelling IRS1 with purified ab40777 at a dilution of 1/100. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
    • Immunocytochemistry - Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)
      Immunocytochemistry - Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)
      This data was developed using ab40777, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labelling IRS1 with purified ab40777 at a dilution of 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used. Control 1: primary antibody (1/250) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000). Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).
    • Western blot - Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)
      Western blot - Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)
      Anti-IRS1 antibody [EP263Y] (ab40777) (purified) + HeLa whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 130 kDa
      Observed band size: 170 kDa why is the actual band size different from the predicted?



      This data was developed using ab40777, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST. 

    • Flow Cytometry (Intracellular) - Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)
      Flow Cytometry (Intracellular) - Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)

      This data was developed using ab40777, the same antibody clone in a different buffer formulation. 

      Intracellular Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling IRS1 with unpurified ab40777 at 1/90 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control. 

       

       

    • Western blot - Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)
      Western blot - Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)
      Anti-IRS1 antibody [EP263Y] (ab40777) at 1/1000 dilution (unpurified) + HeLa cell lysate at 10 µg

      Predicted band size: 130 kDa
      Observed band size: 170 kDa why is the actual band size different from the predicted?



      This data was developed using ab40777, the same antibody clone in a different buffer formulation. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)
      This data was developed using ab40777, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma labelling IRS1 with unpurified ab40777. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    • Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)
      Anti-IRS1 antibody [EP263Y] - BSA and Azide free (ab247286)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab247286? Please let us know so that we can cite the reference in this datasheet.

    ab247286 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab247286.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com