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    irs1-phospho-s307-antibody-ab5599.pdf

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Anti-IRS1 (phospho S307) antibody (ab5599)

  • Datasheet
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Immunocytochemistry - Anti-IRS1 (phospho S307) antibody (ab5599)
  • Western blot - Anti-IRS1 (phospho S307) antibody (ab5599)

Key features and details

  • Rabbit polyclonal to IRS1 (phospho S307)
  • Suitable for: WB, ICC
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-IRS1 (phospho S307) antibody
    See all IRS1 primary antibodies
  • Description

    Rabbit polyclonal to IRS1 (phospho S307)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICCmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, African green monkey
  • Immunogen

    Synthetic peptide corresponding to Human IRS1 aa 300-400 (phospho S307).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Jurkat treated for 20 minutes with 50 ng/ml of TNF alpha, whole cell lysate; NIH/3T3 treated for 20 minutes with 50 ng/ml of TNF alpha, whole cell lysates. ICC: T-47D cells
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Signal Transduction
    • Adapters
    • Cytoplasmic
    • Signal Transduction
    • Growth Factors/Hormones
    • Insulin / Insulin-like
    • Neuroscience
    • Neurology process
    • Metabolism
    • Cancer
    • Signal transduction
    • Other
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Heart disease

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • BALB/3T3 whole cell lysate (ab7901)
  • Recombinant Protein

    • Recombinant Human IRS1 protein (ab70538)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab5599 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 131 kDa (predicted molecular weight: 131 kDa).
ICC
Use a concentration of 1 - 2 µg/ml.
Notes
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 131 kDa (predicted molecular weight: 131 kDa).
ICC
Use a concentration of 1 - 2 µg/ml.

Target

  • Function

    May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
  • Involvement in disease

    Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
  • Sequence similarities

    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • Post-translational
    modifications

    Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
    Phosphorylation of Tyr-896 is required for GRB2-binding.
  • Target information above from: UniProt accession P35568 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3667 Human
    • Entrez Gene: 16367 Mouse
    • Entrez Gene: 25467 Rat
    • Omim: 147545 Human
    • SwissProt: P35568 Human
    • SwissProt: P35569 Mouse
    • SwissProt: P35570 Rat
    • Unigene: 471508 Human
    • Unigene: 4952 Mouse
    • Unigene: 10476 Rat
    see all
  • Alternative names

    • HIRS 1 antibody
    • HIRS1 antibody
    • Insulin receptor substrate 1 antibody
    • IRS 1 antibody
    • IRS-1 antibody
    • IRS1 antibody
    • IRS1_HUMAN antibody
    • OTTHUMP00000164234 antibody
    see all

Images

  • Immunocytochemistry - Anti-IRS1 (phospho S307) antibody (ab5599)
    Immunocytochemistry - Anti-IRS1 (phospho S307) antibody (ab5599)

    Immunocytochemistry analysis of 4% paraformaldehyde-fixed  0.25% Triton™ X-100 permeabilized T-47D cells (Human ductal breast epithelial tumor cell line) staining IRS1 with ab5599 at 1 µg/ml concentration and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution (green). Nuclei were stained with SlowFade® Gold Antifade Mountant with DAPI (blue) and F-actin was stained with Alexa Fluor® 555 Rhodamine Phalloidin (red). Negative control uses no primary antibody. 

  • Western blot - Anti-IRS1 (phospho S307) antibody (ab5599)
    Western blot - Anti-IRS1 (phospho S307) antibody (ab5599)
    All lanes : Anti-IRS1 (phospho S307) antibody (ab5599) at 3 µg/ml

    Lane 1 : Jurkat whole cell lysates
    Lane 2 : Jurkat treated for 20 minutes with 50 ng/ml of TNF alpha whole cell lysate
    Lane 3 : NIH/3T3 whole cell lysates
    Lane 4 : NIH/3T3 treated for 20 minutes with 50 ng/ml of TNF alpha whole cell lysates

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L) secondary antibody, HRP conjugate at 0.4 µg/ml

    Developed using the ECL technique.

    Predicted band size: 131 kDa
    Observed band size: 131 kDa



    Blocking buffer: 5% skimmed milk

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (6)

Publishing research using ab5599? Please let us know so that we can cite the reference in this datasheet.

ab5599 has been referenced in 6 publications.

  • Yun C  et al. Pilose antler peptide promotes osteoblast proliferation, differentiation and mineralization via the insulin signaling pathway. Exp Ther Med 19:923-930 (2020). PubMed: 32010253
  • Li L  et al. Salvianolic acid B prevents body weight gain and regulates gut microbiota and LPS/TLR4 signaling pathway in high-fat diet-induced obese mice. Food Funct 11:8743-8756 (2020). PubMed: 32955050
  • Velickovic N  et al. Modulation of hepatic inflammation and energy-sensing pathways in the rat liver by high-fructose diet and chronic stress. Eur J Nutr 58:1829-1845 (2019). PubMed: 29845385
  • Ma L  et al. Intermittent Hypoxia Composite Abnormal Glucose Metabolism-Mediated Atherosclerosis In Vitro and In Vivo: The Role of SREBP-1. Oxid Med Cell Longev 2019:4862760 (2019). PubMed: 30863480
  • Chang RC  et al. Programmed increases in LXRa induced by paternal alcohol use enhance offspring metabolic adaptation to high-fat diet induced obesity. Mol Metab 30:161-172 (2019). PubMed: 31767168
  • Garabadu D & Krishnamurthy S Diazepam potentiates the antidiabetic, antistress and anxiolytic activities of metformin in type-2 diabetes mellitus with cooccurring stress in experimental animals. Biomed Res Int 2014:693074 (2014). WB ; Rat . PubMed: 24995322

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