Phospho anti-IRS1 (S307) antibody (ab5599)

Product name

Anti-IRS1 (phospho S307) antibody
See all IRS1 primary antibodies
Description

Rabbit polyclonal to IRS1 (phospho S307)
Host species

Rabbit
Tested applications

Suitable for: WBmore details
Species reactivity

Reacts with: Mouse
Predicted to work with: Rat, Human, African green monkey
Immunogen

Synthetic peptide corresponding to Human IRS1 aa 301-315.
Sequence: ESITATS_pPASMVGGK
(Peptide available as ab41770)

Run BLAST with Run BLAST with
Positive control
- 3T3 L1 cells treated with insulin for 10 minutes.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA
Concentration information loading...
Purity

Immunogen affinity purified
Clonality

Polyclonal
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab5599 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		Use a concentration of 2 µg/ml. Detects a band of approximately 170 kDa (predicted molecular weight: 149 kDa).Can be blocked with Human IRS1 (phospho S307) peptide (ab41770).

Function

May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
Involvement in disease

Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
Sequence similarities

Contains 1 IRS-type PTB domain.
Contains 1 PH domain.
Post-translational
modifications

Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
Phosphorylation of Tyr-896 is required for GRB2-binding.
Target information above from: UniProt accession P35568 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 3667 Human
- Entrez Gene: 16367 Mouse
- Entrez Gene: 25467 Rat
- Omim: 147545 Human
- SwissProt: P35568 Human
- SwissProt: P35569 Mouse
- SwissProt: P35570 Rat
- Unigene: 471508 Human
- Unigene: 4952 Mouse
- Unigene: 10476 Rat
see all
Alternative names
- HIRS 1 antibody
- HIRS1 antibody
- Insulin receptor substrate 1 antibody
- IRS 1 antibody
- IRS-1 antibody
- IRS1 antibody
- IRS1_HUMAN antibody
- OTTHUMP00000164234 antibody
see all

Garabadu D & Krishnamurthy S Diazepam potentiates the antidiabetic, antistress and anxiolytic activities of metformin in type-2 diabetes mellitus with cooccurring stress in experimental animals. Biomed Res Int 2014:693074 (2014). WB ; Rat . Read more (PubMed: 24995322) »

Publishing research using ab5599? Please let us know so that we can cite the reference in this datasheet.

Anti-IRS1 (phospho S307) antibody (ab5599)