Overview

  • Product name

    Anti-IRS1 (phospho S312) antibody
    See all IRS1 primary antibodies
  • Description

    Rabbit polyclonal to IRS1 (phospho S312)
  • Host species

    Rabbit
  • Specificity

    ab138456 detects endogenous levels of IRS1 only when phosphorylated at S312.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic phospho-peptide against an internal region surrounding S312 of the Human IRS1 protein.

  • Positive control

    • WB: HepG2 cells extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab138456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 131 kDa.

Target

  • Function

    May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
  • Involvement in disease

    Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
  • Sequence similarities

    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • Post-translational
    modifications

    Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
    Phosphorylation of Tyr-896 is required for GRB2-binding.
  • Information by UniProt
  • Database links

  • Alternative names

    • HIRS 1 antibody
    • HIRS1 antibody
    • Insulin receptor substrate 1 antibody
    • IRS 1 antibody
    • IRS-1 antibody
    • IRS1 antibody
    • IRS1_HUMAN antibody
    • OTTHUMP00000164234 antibody
    see all

Images

  • Anti-IRS1 (phospho S312) antibody (ab138456) at 1/500 dilution + HepG2 cell extracts

    Predicted band size: 131 kDa

References

ab138456 has not yet been referenced specifically in any publications.

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