Key features and details
- Rabbit polyclonal to IRS1 (phospho S794)
- Suitable for: ELISA, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-IRS1 (phospho S794) antibody
See all IRS1 primary antibodies
DescriptionRabbit polyclonal to IRS1 (phospho S794)
SpecificityDetects endogenous levels of IRS-1 only when phosphorylated at serine 794.
Tested applicationsSuitable for: ELISA, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 794 (S-T-SP-S-G).
- Human brain tissue
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide.The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab59305 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
FunctionMay mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
Involvement in diseasePolymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
Sequence similaritiesContains 1 IRS-type PTB domain.
Contains 1 PH domain.
modificationsSerine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
Phosphorylation of Tyr-896 is required for GRB2-binding.
- Information by UniProt
- HIRS 1 antibody
- HIRS1 antibody
- Insulin receptor substrate 1 antibody
ab59305 has not yet been referenced specifically in any publications.