The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Positive (ab5224P) and negative (ab5224N) control peptides for the IRS 1 Receptor phospho tyrosine 896 rabbit polyclonal antibody ab4873.
ab5224P - 1718
ab5224N - 1638
Peptide Content: 80 ± 4%
Quantity: 100ug of each peptide.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituent: 0.1% Trifluoroacetic acid
Insulin receptor substrate 1
May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
Involvement in disease
Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor. Phosphorylation of Tyr-896 is required for GRB2-binding.