Product nameAnti-IRS2 antibody
See all IRS2 primary antibodies
DescriptionRabbit polyclonal to IRS2
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human IRS2 aa 1100-1150. The exact sequence is proprietary. NP_003740.2
Database link: Q9Y4H2
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245387 was affinity purified using an epitope specific to IRS2 immobilized on solid support.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab245387 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 137 kDa.|
FunctionMay mediate the control of various cellular processes by insulin.
Sequence similaritiesContains 1 IRS-type PTB domain.
Contains 1 PH domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm > cytosol.
- Information by UniProt
- Insulin receptor substrate 2 antibody
- IRS 2 antibody
- IRS-2 antibody
All lanes : Anti-IRS2 antibody (ab245387) at 0.04 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 137 kDa
Exposure time: 30 seconds
IRS2 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).
ab245387 used for IP at 3 µg/mg lysate. For WB 1 µg/ml.
Lane 1: ab245387 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Chemiluminescence detection: 10 seconds.
ab245387 has not yet been referenced specifically in any publications.