Overview

  • Product name

  • Description

    Rabbit polyclonal to IRS2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human IRS2 aa 1100-1150. The exact sequence is proprietary. NP_003740.2
    Database link: Q9Y4H2

  • Positive control

    • WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab245387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 137 kDa.

Target

  • Function

    May mediate the control of various cellular processes by insulin.
  • Sequence similarities

    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm > cytosol.
  • Information by UniProt
  • Database links

  • Alternative names

    • Insulin receptor substrate 2 antibody
    • IRS 2 antibody
    • IRS-2 antibody
    • Irs2 antibody
    • IRS2_HUMAN antibody
    see all

Images

  • All lanes : Anti-IRS2 antibody (ab245387) at 0.04 µg/ml

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique.

    Predicted band size: 137 kDa


    Exposure time: 30 seconds
  • IRS2 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).

    ab245387 used for IP at 3 µg/mg lysate. For WB 1 µg/ml.

    Lane 1: ab245387 IP in HeLa whole cell lysate.
    Lane 2: Control IgG.

    Chemiluminescence detection: 10 seconds.

References

ab245387 has not yet been referenced specifically in any publications.

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