Key features and details
- Rabbit polyclonal to IRS2
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-IRS2 antibody
See all IRS2 primary antibodies
DescriptionRabbit polyclonal to IRS2
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human IRS2 aa 1100-1150. The exact sequence is proprietary. NP_003740.2
Database link: Q9Y4H2
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245387 was affinity purified using an epitope specific to IRS2 immobilized on solid support.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab245387 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 137 kDa.|
FunctionMay mediate the control of various cellular processes by insulin.
Sequence similaritiesContains 1 IRS-type PTB domain.
Contains 1 PH domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm > cytosol.
- Information by UniProt
- Insulin receptor substrate 2 antibody
- IRS 2 antibody
- IRS-2 antibody
All lanes : Anti-IRS2 antibody (ab245387) at 0.04 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 137 kDa
Exposure time: 30 seconds
IRS2 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).
ab245387 used for IP at 3 µg/mg lysate. For WB 1 µg/ml.
Lane 1: ab245387 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Chemiluminescence detection: 10 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245387 has not yet been referenced specifically in any publications.