Product nameAnti-IRS2 antibody [EP903Y]
See all IRS2 primary antibodies
DescriptionRabbit monoclonal [EP903Y] to IRS2
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IPmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Human
A synthetic peptide corresponding to residues near the N-terminus of human IRS2.
- 293 cell lysate + insulin; human cervical carcinoma tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA
PurityTissue culture supernatant
Purification notesThis antibody has not been purified. It is provided in tissue culture supernatant and storage buffer.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab46811 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100000. Predicted molecular weight: 137 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/250.|
FunctionMay mediate the control of various cellular processes by insulin.
Sequence similaritiesContains 1 IRS-type PTB domain.
Contains 1 PH domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm > cytosol.
- Information by UniProt
- Insulin receptor substrate 2 antibody
- IRS 2 antibody
- IRS-2 antibody
Anti-IRS2 antibody [EP903Y] (ab46811) at 1/100000 dilution + 293 cell lysate at 10ug/lane, plus insulin
Goat-anti rabbit, HRP labelled, at 1/2000 dilution
Predicted band size: 137 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa (possible non-specific binding)
The 50 kDa band is most likely the heavy chain. This appears when using human tissue or samples which may contain traces of blood. There are secondary antibodies such as Tru-Blot that can eliminate this heavy chain band.
This image shows human cervical carcinoma stained with ab46811 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This product has been referenced in:
- Pang YL et al. Comparative medical characteristics of ZDF-T2DM rats during the course of development to late stage disease. Animal Model Exp Med 1:203-211 (2018). Read more (PubMed: 30891566) »
- Lopez R et al. Hyperglycemia enhances the proliferation of non-tumorigenic and malignant mammary epithelial cells through increased leptin/IGF1R signaling and activation of AKT/mTOR. PLoS One 8:e79708 (2013). WB ; Human . Read more (PubMed: 24260287) »