RabMAb

Anti-IRS2 antibody [EP903Y] (ab46811)

Submit a review Submit a question References (2)

Overview

  • Product name

    Anti-IRS2 antibody [EP903Y]
    See all IRS2 primary antibodies

  • Description

    Rabbit monoclonal [EP903Y] to IRS2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human

  • Immunogen

    A synthetic peptide corresponding to residues near the N-terminus of human IRS2.

  • Positive control

    • 293 cell lysate + insulin; human cervical carcinoma tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab46811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100000. Predicted molecular weight: 137 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
IP 1/50.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Function

      May mediate the control of various cellular processes by insulin.

    • Sequence similarities

      Contains 1 IRS-type PTB domain.
      Contains 1 PH domain.

    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.

    • Cellular localization

      Cytoplasm > cytosol.
    • Information by UniProt

    • Database links

      • Alternative names

        • Insulin receptor substrate 2 antibody
        • IRS 2 antibody
        • IRS-2 antibody
        • Irs2 antibody
        • IRS2_HUMAN antibody
        see all

      Images

      • Western blot - Anti-IRS2 antibody [EP903Y] (ab46811)
        Western blot - Anti-IRS2 antibody [EP903Y] (ab46811)
        Anti-IRS2 antibody [EP903Y] (ab46811) at 1/100000 dilution + 293 cell lysate at 10ug/lane, plus insulin

        Secondary
        Goat-anti rabbit, HRP labelled, at 1/2000 dilution

        Predicted band size: 137 kDa
        Observed band size: 160 kDa
        why is the actual band size different from the predicted?
        Additional bands at: 50 kDa (possible non-specific binding)



        The 50 kDa band is most likely the heavy chain. This appears when using human tissue or samples which may contain traces of blood. There are secondary antibodies such as Tru-Blot that can eliminate this heavy chain band.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS2 antibody [EP903Y] (ab46811)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS2 antibody [EP903Y] (ab46811)

        This image shows human cervical carcinoma stained with ab46811 at 1/50 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      References

      This product has been referenced in:

      • Pang YL  et al. Comparative medical characteristics of ZDF-T2DM rats during the course of development to late stage disease. Animal Model Exp Med 1:203-211 (2018). Read more (PubMed: 30891566) »
      • Lopez R  et al. Hyperglycemia enhances the proliferation of non-tumorigenic and malignant mammary epithelial cells through increased leptin/IGF1R signaling and activation of AKT/mTOR. PLoS One 8:e79708 (2013). WB ; Human . Read more (PubMed: 24260287) »
      See all 2 Publications for this product

      Publishing research using ab46811? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab46811.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com