Anti-IRS2 antibody [EP903Y] (ab46811)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-IRS2 antibody [EP903Y]
See all IRS2 primary antibodies -
DescriptionRabbit monoclonal [EP903Y] to IRS2
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IPmore details
Unsuitable for: Flow Cyt -
Species reactivityReacts with: Human
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Immunogen
A synthetic peptide corresponding to residues near the N-terminus of human IRS2.
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Positive control
- 293 cell lysate + insulin; human cervical carcinoma tissue.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA -
PurityTissue culture supernatant
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Purification notesThis antibody has not been purified. It is provided in tissue culture supernatant and storage buffer.
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ClonalityMonoclonal
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Clone numberEP903Y
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab46811 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/100000. Predicted molecular weight: 137 kDa. | |
| IHC-P | 1/50 - 1/100. | |
| ICC/IF | 1/100 - 1/250. | |
| IP | 1/50. |
Application notes
Is unsuitable for Flow Cyt.
Target
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FunctionMay mediate the control of various cellular processes by insulin.
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Sequence similaritiesContains 1 IRS-type PTB domain.
Contains 1 PH domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localizationCytoplasm > cytosol.
- Information by UniProt
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Database links
- Entrez Gene: 8660 Human
- Omim: 600797 Human
- SwissProt: Q9Y4H2 Human
- Unigene: 442344 Human
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Alternative names
- Insulin receptor substrate 2 antibody
- IRS 2 antibody
- IRS-2 antibody
see all
Images
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![Western blot - Anti-IRS2 antibody [EP903Y] (ab46811)](https://www.abcam.com/ps/products/46/ab46811/Images/ab46811_WB.jpg)
Western blot - Anti-IRS2 antibody [EP903Y] (ab46811)Anti-IRS2 antibody [EP903Y] (ab46811) at 1/100000 dilution + 293 cell lysate at 10ug/lane, plus insulin
Secondary
Goat-anti rabbit, HRP labelled, at 1/2000 dilution
Predicted band size: 137 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa (possible non-specific binding)
The 50 kDa band is most likely the heavy chain. This appears when using human tissue or samples which may contain traces of blood. There are secondary antibodies such as Tru-Blot that can eliminate this heavy chain band. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS2 antibody [EP903Y] (ab46811)](https://www.abcam.com/ps/products/46/ab46811/Images/ab46811_IHC.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS2 antibody [EP903Y] (ab46811)This image shows human cervical carcinoma stained with ab46811 at 1/50 dilution.
Datasheets and documents
References
This product has been referenced in:
- Lopez R et al. Hyperglycemia enhances the proliferation of non-tumorigenic and malignant mammary epithelial cells through increased leptin/IGF1R signaling and activation of AKT/mTOR. PLoS One 8:e79708 (2013). WB ; Human . Read more (PubMed: 24260287) »
