Recombinant Anti-IRS4 antibody [EP907Y] (ab52622)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP907Y] to IRS4
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-IRS4 antibody [EP907Y]
See all IRS4 primary antibodies -
Description
Rabbit monoclonal [EP907Y] to IRS4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293 whole cell lysate; IP: HEK-293 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP907Y -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52622 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/10000. Detects a band of approximately 133 kDa (predicted molecular weight: 133 kDa).
For unpurified use at 1/5000 dilution |
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IP |
1/20.
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Notes |
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WB
1/10000. Detects a band of approximately 133 kDa (predicted molecular weight: 133 kDa). For unpurified use at 1/5000 dilution |
IP
1/20. |
Target
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Relevance
IRS4 (Insulin receptor substrate 4) is a cytoplasmic protein that contains many potential tyrosine and serine/threonine phosphorylation sites. Tyrosine-phosphorylated IRS4 protein has been shown to associate with cytoplasmic signaling molecules that contain SH2 domains. The IRS4 protein is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation. -
Cellular localization
Cell membrane; Peripheral membrane protein; Cytoplasmic side. -
Database links
- Entrez Gene: 8471 Human
- Omim: 603510 Human
- SwissProt: O14654 Human
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Alternative names
- 160 kDa phosphotyrosine protein antibody
- Insulin receptor substrate 4 antibody
- IRS 4 antibody
- PY160 antibody
Images
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Anti-IRS4 antibody [EP907Y] (ab52622) at 1/10000 dilution (Purified) + HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 133 kDa
Observed band size: 133 kDaBlocking buffer: 5% NFDM/TBST
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Purified ab52622 at 1/20 dilution (0.5µg) immunoprecipitating IRS4 in HEK-293 whole cell lysate.
Lane 1 (input): HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab52622 + HEK-293 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52622 in HEK-293 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 133 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab52622 has been referenced in 8 publications.
- Yang F et al. Long Noncoding RNA LINC00173 Promotes the Malignancy of Melanoma by Promoting the Expression of IRS4 Through Competitive Binding to microRNA-493. Cancer Manag Res 12:3131-3144 (2020). PubMed: 32440211
- Li X et al. Phosphorylation of IRS4 by CK1?2 promotes its degradation by CHIP through the ubiquitin/lysosome pathway. Theranostics 8:3643-3653 (2018). PubMed: 30026872
- Weischenfeldt J et al. Pan-cancer analysis of somatic copy-number alterations implicates IRS4 and IGF2 in enhancer hijacking. Nat Genet 49:65-74 (2017). PubMed: 27869826
- Cui A et al. Downregulation of miR-493 promoted melanoma proliferation by suppressing IRS4 expression. Tumour Biol 39:1010428317701640 (2017). PubMed: 28475006
- Homma Y et al. Insulin receptor substrate-4 binds to Slingshot-1 phosphatase and promotes cofilin dephosphorylation. J Biol Chem 289:26302-13 (2014). PubMed: 25100728
- Chai B et al. A novel transcript is up-regulated by fasting in the hypothalamus and enhances insulin signaling. J Neuroendocrinol : (2012). PubMed: 22935015
- Mertens F et al. The t(X;6) in subungual exostosis results in transcriptional deregulation of the gene for insulin receptor substrate 4. Int J Cancer 128:487-91 (2011). PubMed: 20340132
- Karrman K et al. The t(X;7)(q22;q34) in paediatric T-cell acute lymphoblastic leukaemia results in overexpression of the insulin receptor substrate 4 gene through illegitimate recombination with the T-cell receptor beta locus. Br J Haematol 144:546-51 (2009). WB ; Human . PubMed: 19055661