• Product name
  • Description
    Rabbit polyclonal to IRSp53
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human IRSp53 aa 1-179. The exact sequence is proprietary.
    Database link: Q9UQB8

  • Positive control
    • SK N SH and mouse brain whole cell lysates; A431 cells; Human Cal27 xenograft tissue.
  • General notes

    Protein previously known as BAIAP2.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab126057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 61 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.


  • Function
    Adapter protein that links membrane-bound small G-proteins to cytoplasmic effector proteins. Necessary for CDC42-mediated reorganization of the actin cytoskeleton and for RAC1-mediated membrane ruffling. Involved in the regulation of the actin cytoskeleton by WASF family members and the Arp2/3 complex. Plays a role in neurite growth. Acts syngeristically with ENAH to promote filipodia formation.
  • Tissue specificity
    Isoform 1 and isoform 4 are expressed almost exclusively in brain. Isoform 4 is barely detectable in placenta, prostate and testis. A short isoform is ubiquitous, with the highest expression in liver, prostate, testis and placenta.
  • Sequence similarities
    Contains 1 IMD (IRSp53/MIM homology) domain.
    Contains 1 SH3 domain.
  • Domain
    The IMD domain forms a coiled coil. The isolated domain can induce actin bundling and filopodia formation. In the absence of G-proteins intramolecular interaction between the IMD and the SH3 domain gives rise to an auto-inhibited state of the protein. Interaction of the IMD with RAC1 or CDC42 leads to activation.
    The SH3 domain interacts with ATN1, BAI1, WASF1, WASF2, SHANK1, DIAPH1 and ENAH.
  • Post-translational
    Phosphorylated on tyrosine residues by INSR in response to insulin treatment.
  • Cellular localization
    Cytoplasm. Membrane. Cell projection > filopodium. Cell projection > ruffle. Detected throughout the cytoplasm in the absence of specific binding partners. Detected in filopodia and close to membrane ruffles.
  • Information by UniProt
  • Database links
  • Alternative names
    • BAI-associated protein 2 antibody
    • BAI1 associated protein 2 antibody
    • BAI1-associated protein 2 antibody
    • BAIAP 2 antibody
    • Baiap2 antibody
    • BAIP2_HUMAN antibody
    • BAP2 antibody
    • Brain-specific angiogenesis inhibitor 1-associated protein 2 antibody
    • Fas ligand-associated factor 3 antibody
    • FLAF3 antibody
    • Insulin receptor substrate p53 antibody
    • Insulin receptor substrate p53/p58 antibody
    • Insulin receptor substrate protein of 53 kDa antibody
    • IRS-58 antibody
    • IRSp53 antibody
    • IRSp53/58 antibody
    • Protein BAP2 antibody
    see all


  • Anti-IRSp53 antibody (ab126057) at 1/2000 dilution + SK N SH whole cell lysate at 30 µg

    Predicted band size: 61 kDa

    7.5% SDS PAGE
  • Anti-IRSp53 antibody (ab126057) at 1/10000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 61 kDa

    7.5% SDS PAGE
  • ab126057, at 1/500 dilution, staining IRSp53 in paraffin-embedded human Cal27 xenograft tissue, by Immunohistochemistry.

  • ab126057, at 1/500 dilution, staining IRSp53 in methanol-fixed A431 cells by Immunofluorescence (green).
    Alpha-tubulin filaments are stained in red.


ab126057 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Merci de confirmer ces détails et de votre coopération. Les détails fournis nous permettent de suivre de près la qualité de nos produits.

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Merci beaucoup pour toutes ces informations détaillées qui nous sont très utiles pour identifier l'origine des problèmes rencontrés avec ab92312 et ab126057.

Je n'ai rien à modifier à votre prot...

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Je suis désolée d'apprendre que les anticorps ab92312 et ab126057 ne vous donnent pas de bons résultats en WB.

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