Overview

  • Product name

  • Description

    Rabbit polyclonal to IRSp53
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human IRSp53 aa 492-518 (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q9UQB8-5
    (Peptide available as ab170411)

  • Positive control

    • Mouse brain tissue lysate, A375 cell lysate and human breast carcinoma tissue.
  • General notes

    Protein previously known as BAIAP2.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    This antibody was purified through a protein G column and eluted out with both high and low pH buffers. It was neutralized immediately after elution, followed by dialysis against PBS.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab37542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
ELISA 1/1000.
IHC-P Use at an assay dependent concentration.

Target

  • Function

    Adapter protein that links membrane-bound small G-proteins to cytoplasmic effector proteins. Necessary for CDC42-mediated reorganization of the actin cytoskeleton and for RAC1-mediated membrane ruffling. Involved in the regulation of the actin cytoskeleton by WASF family members and the Arp2/3 complex. Plays a role in neurite growth. Acts syngeristically with ENAH to promote filipodia formation.
  • Tissue specificity

    Isoform 1 and isoform 4 are expressed almost exclusively in brain. Isoform 4 is barely detectable in placenta, prostate and testis. A short isoform is ubiquitous, with the highest expression in liver, prostate, testis and placenta.
  • Sequence similarities

    Contains 1 IMD (IRSp53/MIM homology) domain.
    Contains 1 SH3 domain.
  • Domain

    The IMD domain forms a coiled coil. The isolated domain can induce actin bundling and filopodia formation. In the absence of G-proteins intramolecular interaction between the IMD and the SH3 domain gives rise to an auto-inhibited state of the protein. Interaction of the IMD with RAC1 or CDC42 leads to activation.
    The SH3 domain interacts with ATN1, BAI1, WASF1, WASF2, SHANK1, DIAPH1 and ENAH.
  • Post-translational
    modifications

    Phosphorylated on tyrosine residues by INSR in response to insulin treatment.
  • Cellular localization

    Cytoplasm. Membrane. Cell projection > filopodium. Cell projection > ruffle. Detected throughout the cytoplasm in the absence of specific binding partners. Detected in filopodia and close to membrane ruffles.
  • Information by UniProt
  • Database links

  • Alternative names

    • BAI-associated protein 2 antibody
    • BAI1 associated protein 2 antibody
    • BAI1-associated protein 2 antibody
    • BAIAP 2 antibody
    • Baiap2 antibody
    • BAIP2_HUMAN antibody
    • BAP2 antibody
    • Brain-specific angiogenesis inhibitor 1-associated protein 2 antibody
    • Fas ligand-associated factor 3 antibody
    • FLAF3 antibody
    • Insulin receptor substrate p53 antibody
    • Insulin receptor substrate p53/p58 antibody
    • Insulin receptor substrate protein of 53 kDa antibody
    • IRS-58 antibody
    • IRSp53 antibody
    • IRSp53/58 antibody
    • Protein BAP2 antibody
    see all

Images

  • All lanes : Anti-IRSp53 antibody (ab37542) at 1/100 dilution

    Lane 1 : Mouse brain cell lysate
    Lane 2 : A375 cell lysate

    Predicted band size: 57 kDa
    Observed band size: 57 kDa

  • Formalin-fixed and paraffin-embedded Human breast cancer tissue reacted with ab37542, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

    This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

I have contacted the lab who have agreed to add the blocking peptide for ab37542 to the catalogue. The product is called BAIAP2 peptide, ab170411.

As for EXPOSE Mouse and Rabbit Specific HRP/DAB Detection IHC Kit, I can confirm that it does have the same sensitivity as the EXPOSE Rabbit Specific HRP/DAB Detection IHC Kit

Read More
Application
Western blot
Sample
Human Cell lysate - whole cell (human HEK293 cell)
Gel Running Conditions
Reduced Denaturing (8% Gel)
Loading amount
20 µg
Specification
human HEK293 cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Mar 16 2012

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.01 M Tris, pH 10
Permeabilization
Yes - 0.3% Triton
Specification
brain
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Sep 09 2011

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Aug 24 2011

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