• Product name
    Anti-IRSp53 antibody - C-terminal
    See all IRSp53 primary antibodies
  • Description
    Goat polyclonal to IRSp53 - C-terminal
  • Host species
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Dog, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human IRSp53 aa 512-520 (C terminal).


    (Peptide available as ab23256)

  • Positive control
    • WB: Human brain lysate.
  • General notes

    This antibody is expected to recognise the human isoform 1 only of this protein.

    Protein previously known as BAIAP2.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.05% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    This antibody is expected to recognise the human isoform 1 only of this protein.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab15697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).Can be blocked with Human IRSp53 peptide (ab23256).


  • Function
    Adapter protein that links membrane-bound small G-proteins to cytoplasmic effector proteins. Necessary for CDC42-mediated reorganization of the actin cytoskeleton and for RAC1-mediated membrane ruffling. Involved in the regulation of the actin cytoskeleton by WASF family members and the Arp2/3 complex. Plays a role in neurite growth. Acts syngeristically with ENAH to promote filipodia formation.
  • Tissue specificity
    Isoform 1 and isoform 4 are expressed almost exclusively in brain. Isoform 4 is barely detectable in placenta, prostate and testis. A short isoform is ubiquitous, with the highest expression in liver, prostate, testis and placenta.
  • Sequence similarities
    Contains 1 IMD (IRSp53/MIM homology) domain.
    Contains 1 SH3 domain.
  • Domain
    The IMD domain forms a coiled coil. The isolated domain can induce actin bundling and filopodia formation. In the absence of G-proteins intramolecular interaction between the IMD and the SH3 domain gives rise to an auto-inhibited state of the protein. Interaction of the IMD with RAC1 or CDC42 leads to activation.
    The SH3 domain interacts with ATN1, BAI1, WASF1, WASF2, SHANK1, DIAPH1 and ENAH.
  • Post-translational
    Phosphorylated on tyrosine residues by INSR in response to insulin treatment.
  • Cellular localization
    Cytoplasm. Membrane. Cell projection > filopodium. Cell projection > ruffle. Detected throughout the cytoplasm in the absence of specific binding partners. Detected in filopodia and close to membrane ruffles.
  • Information by UniProt
  • Database links
  • Alternative names
    • BAI-associated protein 2 antibody
    • BAI1 associated protein 2 antibody
    • BAI1-associated protein 2 antibody
    • BAIAP 2 antibody
    • Baiap2 antibody
    • BAIP2_HUMAN antibody
    • BAP2 antibody
    • Brain-specific angiogenesis inhibitor 1-associated protein 2 antibody
    • Fas ligand-associated factor 3 antibody
    • FLAF3 antibody
    • Insulin receptor substrate p53 antibody
    • Insulin receptor substrate p53/p58 antibody
    • Insulin receptor substrate protein of 53 kDa antibody
    • IRS-58 antibody
    • IRSp53 antibody
    • IRSp53/58 antibody
    • Protein BAP2 antibody
    see all


  • Anti-IRSp53 antibody - C-terminal (ab15697) at 1 µg/ml + Lysates prepared from human brain at 35 µg

    Predicted band size: 60 kDa

    ab15697 staining (1µg/ml) of human brain lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence.


This product has been referenced in:
  • Goodwin JM  et al. An AMPK-independent signaling pathway downstream of the LKB1 tumor suppressor controls Snail1 and metastatic potential. Mol Cell 55:436-50 (2014). Read more (PubMed: 25042806) »
  • Cohen D  et al. The serine/threonine kinase Par1b regulates epithelial lumen polarity via IRSp53-mediated cell-ECM signaling. J Cell Biol 192:525-40 (2011). WB ; Dog . Read more (PubMed: 21282462) »
See all 2 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Mouse Tissue lysate - whole (Whole adult brain lysate)
Whole adult brain lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

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Verified customer

Submitted Feb 08 2006


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