Overview

  • Product name

    Anti-IRSp53 antibody - C-terminal
    See all IRSp53 primary antibodies
  • Description

    Goat polyclonal to IRSp53 - C-terminal
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow, Chinese hamster
  • Immunogen

    Synthetic peptide corresponding to Human IRSp53 aa 509-520 (C terminal) (Cysteine residue). NP_006331.1
    Sequence:

    SMSSADVEVARF


    Database link: Q9UQB8-5

  • Positive control

    • Human brain cortex tissue. Human brain cerebellum and frontal cortex lysates.
  • General notes

    Protein previously known as BAIAP2.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab189190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 57 kDa.
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Adapter protein that links membrane-bound small G-proteins to cytoplasmic effector proteins. Necessary for CDC42-mediated reorganization of the actin cytoskeleton and for RAC1-mediated membrane ruffling. Involved in the regulation of the actin cytoskeleton by WASF family members and the Arp2/3 complex. Plays a role in neurite growth. Acts syngeristically with ENAH to promote filipodia formation.
  • Tissue specificity

    Isoform 1 and isoform 4 are expressed almost exclusively in brain. Isoform 4 is barely detectable in placenta, prostate and testis. A short isoform is ubiquitous, with the highest expression in liver, prostate, testis and placenta.
  • Sequence similarities

    Contains 1 IMD (IRSp53/MIM homology) domain.
    Contains 1 SH3 domain.
  • Domain

    The IMD domain forms a coiled coil. The isolated domain can induce actin bundling and filopodia formation. In the absence of G-proteins intramolecular interaction between the IMD and the SH3 domain gives rise to an auto-inhibited state of the protein. Interaction of the IMD with RAC1 or CDC42 leads to activation.
    The SH3 domain interacts with ATN1, BAI1, WASF1, WASF2, SHANK1, DIAPH1 and ENAH.
  • Post-translational
    modifications

    Phosphorylated on tyrosine residues by INSR in response to insulin treatment.
  • Cellular localization

    Cytoplasm. Membrane. Cell projection > filopodium. Cell projection > ruffle. Detected throughout the cytoplasm in the absence of specific binding partners. Detected in filopodia and close to membrane ruffles.
  • Information by UniProt
  • Database links

  • Alternative names

    • BAI-associated protein 2 antibody
    • BAI1 associated protein 2 antibody
    • BAI1-associated protein 2 antibody
    • BAIAP 2 antibody
    • Baiap2 antibody
    • BAIP2_HUMAN antibody
    • BAP2 antibody
    • Brain-specific angiogenesis inhibitor 1-associated protein 2 antibody
    • Fas ligand-associated factor 3 antibody
    • FLAF3 antibody
    • Insulin receptor substrate p53 antibody
    • Insulin receptor substrate p53/p58 antibody
    • Insulin receptor substrate protein of 53 kDa antibody
    • IRS-58 antibody
    • IRSp53 antibody
    • IRSp53/58 antibody
    • Protein BAP2 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human brain cortex tissue labeling IRSp53 with ab189190 at 3.75 µg/ml.

  • Anti-IRSp53 antibody - C-terminal (ab189190) at 1 µg/ml + human brain cerebellum lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 57 kDa



    An additional band of unknown identity was also consistently observed at 150-170 kDa. This band was successfully blocked by incubation with the immunizing peptide.

References

ab189190 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab189190.
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