Key features and details
- Goat polyclonal to IRSp53 - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-IRSp53 antibody - C-terminal
See all IRSp53 primary antibodies
DescriptionGoat polyclonal to IRSp53 - C-terminal
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow, Chinese hamster
- Human brain cortex tissue. Human brain cerebellum and frontal cortex lysates.
Protein previously known as BAIAP2.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab189190 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 57 kDa.|
|IHC-P||Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionAdapter protein that links membrane-bound small G-proteins to cytoplasmic effector proteins. Necessary for CDC42-mediated reorganization of the actin cytoskeleton and for RAC1-mediated membrane ruffling. Involved in the regulation of the actin cytoskeleton by WASF family members and the Arp2/3 complex. Plays a role in neurite growth. Acts syngeristically with ENAH to promote filipodia formation.
Tissue specificityIsoform 1 and isoform 4 are expressed almost exclusively in brain. Isoform 4 is barely detectable in placenta, prostate and testis. A short isoform is ubiquitous, with the highest expression in liver, prostate, testis and placenta.
Sequence similaritiesContains 1 IMD (IRSp53/MIM homology) domain.
Contains 1 SH3 domain.
DomainThe IMD domain forms a coiled coil. The isolated domain can induce actin bundling and filopodia formation. In the absence of G-proteins intramolecular interaction between the IMD and the SH3 domain gives rise to an auto-inhibited state of the protein. Interaction of the IMD with RAC1 or CDC42 leads to activation.
The SH3 domain interacts with ATN1, BAI1, WASF1, WASF2, SHANK1, DIAPH1 and ENAH.
modificationsPhosphorylated on tyrosine residues by INSR in response to insulin treatment.
Cellular localizationCytoplasm. Membrane. Cell projection > filopodium. Cell projection > ruffle. Detected throughout the cytoplasm in the absence of specific binding partners. Detected in filopodia and close to membrane ruffles.
- Information by UniProt
- BAI-associated protein 2 antibody
- BAI1 associated protein 2 antibody
- BAI1-associated protein 2 antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human brain cortex tissue labeling IRSp53 with ab189190 at 3.75 µg/ml.
Anti-IRSp53 antibody - C-terminal (ab189190) at 1 µg/ml + human brain cerebellum lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 57 kDa
An additional band of unknown identity was also consistently observed at 150-170 kDa. This band was successfully blocked by incubation with the immunizing peptide.
ab189190 has not yet been referenced specifically in any publications.