Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13139] to IRX1 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Human
Product nameAnti-IRX1 antibody [EPR13139] - BSA and Azide free
See all IRX1 primary antibodies
DescriptionRabbit monoclonal [EPR13139] to IRX1 - BSA and Azide free
Tested applicationsSuitable for: IP, WBmore details
Unsuitable for: ICC or IHC-P
Species reactivityReacts with: Human
Synthetic peptide within Human IRX1 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: P78414
ab250260 is the carrier-free version of ab180860 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab250260 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab250260 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 50 kDa.|
RelevanceIRX1 is a member of the Iroquois homeobox gene family. The genes from this family have multiple roles in vertebrate development. The gene encoding IRX1 is expressed in the brain, lung and gut during specific stages of mouse development.
- Homeodomain protein IRXA1 antibody
- Iroquois Homeobox Protein 1 antibody
- Iroquois-class homeodomain protein IRX-1 antibody
This data was developed using ab180860, the same antibody clone in a different buffer formulation.
Western blot analysis of immunoprecipitation pellet from (1) Human stomach lysate or (2) 1X PBS (negative control) immunoprecipitated using ab180860 at 1/10 dilution; HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of Rabbit IgG.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab250260 has not yet been referenced specifically in any publications.