Key features and details
- Mouse polyclonal to IRX2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-IRX2 antibody
See all IRX2 primary antibodies
DescriptionMouse polyclonal to IRX2
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant full length protein within Human IRX2. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
- Hela cells and IRX2 transfected 293T cell lysate
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.4
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein A purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72975 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Protein A purify ab72975 before starting ICC/IF procedure.
WB: 1/500 - 1/1000. Predicted molecular weight: 52 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Sequence similaritiesBelongs to the TALE/IRO homeobox family.
Contains 1 homeobox DNA-binding domain.
- Information by UniProt
- Homeodomain protein IRXA2 antibody
- Iroquois homeobox 2 antibody
- Iroquois homeobox protein 2 antibody
All lanes : Anti-IRX2 antibody (ab72975) at 1/500 dilution
Lane 1 : IRX2 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
HeLa cells with Protein A purified ab72975 at 10ug/ml.
ab72975 has been referenced in 2 publications.