Key features and details
- Rabbit polyclonal to IRX5
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-IRX5 antibody
See all IRX5 primary antibodies
DescriptionRabbit polyclonal to IRX5
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
- ICC/IF: HaCaT cells. WB: U-251MG cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab254623 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 22 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionEstablishes the cardiac repolarization gradient by its repressive actions on the KCND2 potassium-channel gene. Required for retinal cone bipolar cell differentiation. May regulate contrast adaptation in the retina and control specific aspects of visual function in circuits of the mammalian retina (By similarity). Could be involved in the regulation of both the cell cycle and apoptosis in prostate cancer cells.
Sequence similaritiesBelongs to the TALE/IRO homeobox family.
Contains 1 homeobox DNA-binding domain.
- Information by UniProt
- Homeodomain protein IRX-2A antibody
- Homeodomain protein IRXB2 antibody
- Iroquois homeobox protein 5 antibody
PFA fixed, Triton X-100 permeabilized HaCaT (Human keratinocyte cell line) cells labeling IRX5 using ab254623 at 4µg/ml (green) in ICC/IF.
Lanes 2-3 : Anti-IRX5 antibody (ab254623) at 0.4 µg/ml
Lane 1 : DNA Ladder
Lane 2 : RT4 (Human urinary bladder cancer cell line) whole cell lysate
Lane 3 : U-251MG sp whole cell lysate
Predicted band size: 22 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab254623 has not yet been referenced specifically in any publications.