Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride
Concentration information loading...
PurityProtein G purified
Purification notesAffinity purified by protein G column chromatography from culture supernatant of hybridoma. Antibody solution was filtered through a 0.22 µm membrane.
Our Abpromise guarantee covers the use of ab50774 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. Detects a band of approximately 32 kDa (predicted molecular weight: 54 kDa).|
FunctionEstablishes the cardiac repolarization gradient by its repressive actions on the KCND2 potassium-channel gene. Required for retinal cone bipolar cell differentiation. May regulate contrast adaptation in the retina and control specific aspects of visual function in circuits of the mammalian retina (By similarity). Could be involved in the regulation of both the cell cycle and apoptosis in prostate cancer cells.
Sequence similaritiesBelongs to the TALE/IRO homeobox family.
Contains 1 homeobox DNA-binding domain.
- Information by UniProt
- Homeodomain protein IRX-2A antibody
- Homeodomain protein IRXB2 antibody
- Iroquois homeobox protein 5 antibody
Lane 1 : Marker
Lane 2 : Anti-IRX5 antibody [IRX5C10G5] (ab50774)
Lane 2 : Immunizing recombinant peptide
Predicted band size: 54 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
ab50774 has not yet been referenced specifically in any publications.