Anti-IRX7 antibody (ab234984)
Key features and details
- Rabbit polyclonal to IRX7
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-IRX7 antibody -
Description
Rabbit polyclonal to IRX7 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment corresponding to Human IRX7 aa 250-400.
Database link: P78412 -
Positive control
- WB: A549 whole cell lysate; Rat heart lysate. IHC-P: Human cervical cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab234984 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/500.
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WB |
1/500 - 1/5000. Predicted molecular weight: 48 kDa.
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Notes |
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IHC-P
1/200 - 1/500. |
WB
1/500 - 1/5000. Predicted molecular weight: 48 kDa. |
Target
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Sequence similarities
Belongs to the TALE/IRO homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 79190 Human
- Entrez Gene: 307715 Rat
- Omim: 606196 Human
- SwissProt: P78412 Human
- Unigene: 369907 Human
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Alternative names
- Homeodomain protein IRXB3 antibody
- Iroquois class Homeodomain Protein 6 antibody
- Iroquois class homeodomain protein IRX 6 antibody
see all
Images
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All lanes : Anti-IRX7 antibody (ab234984) at 1/500 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : Rat heart lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 48 kDa -
Paraffin-embedded human cervical cancer tissue stained for IRX7 using ab234984 at 1/200 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab234984 has not yet been referenced specifically in any publications.